Expression and Purification of Active WW Domains of FBP11/HYPA and FBP28/CA150

Yusuke Kato; Yoriko Sawano; Masaru Tanokura

doi:10.2174/092986606775101670

ISSN: 0929-8665
E-ISSN: 1875-5305

Expression and Purification of Active WW Domains of FBP11/HYPA and FBP28/CA150
Authors: Yusuke Kato¹, Yoriko Sawano² and Masaru Tanokura³
View Affiliations Hide Affiliations

¹ Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. ² Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. ³ Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Source: Protein and Peptide Letters, Volume 13, Issue 2, Feb 2006, p. 197 - 201
DOI: https://doi.org/10.2174/092986606775101670
- Available online: 01 Feb 2006

Abstract

Production of GST-fused WW domains of FBP proteins was increased using the bubbling cultivation method for E. coli. Purified WW domains of FBP11 and FBP28 bound A PL motif peptide with dissociation constants (KD) of 248 ± 27 and 1880 ± 280 μM, respectively.

Article metrics loading...

/content/journals/ppl/10.2174/092986606775101670

2006-02-01

2025-05-23

From This Site

/content/journals/ppl/10.2174/092986606775101670

dcterms_title,dcterms_subject,pub_keyword

-contentType:Contributor -contentType:Concept -contentType:Institution

10

5

Full text loading...

/content/journals/ppl/10.2174/092986606775101670

Article Type: Research Article

Keyword(s): air pump; reverse phase chromatography; surface plasmon resonance; WW domain

Expression and Purification of Active WW Domains of FBP11/HYPA and FBP28/CA150

Abstract

From This Site

Most Read This Month

Most Cited Most Cited RSS feed

The LL-37 Antimicrobial Peptide as a Treatment for Systematic Infection of Acinetobacter baumannii in a Mouse Model

FN1 Promotes Thyroid Carcinoma Cell Proliferation and Metastasis by Activating the NF-b Pathway

Wogonin Restrains the Malignant Progression of Lung Cancer Through Modulating MMP1 and PI3K/AKT Signaling Pathway

s A Temporin Derived Peptide Showing Antibacterial and Antibiofilm Activities against Staphylococcus aureus

Upregulation of Connexins in the Rat Hippocampal and Cortical Neurons Following Blockade of NMDA Receptors During Postnatal Development

Long Non-coding RNA LINC00473 Promotes Breast Cancer Progression via miR-424-5p/CCNE1 Pathway

Protease Inhibitors (PIs): Candidate Molecules for Crop Protection Formulations against Necrotrophs

Preparation of IgE Antibody and Distribution of IgE⁺ Secretory Cells in the Palatine Tonsil of Bactrian Camel

Different VH3-binding Protein A Resins Show Comparable VH3-binding Mediated Byproduct Separation Capabilities Despite Having Varied Dynamic Binding Capacities Towards A VH3 Fab

Immunogenicity and Neutralization Potential of Recombinant Chimeric Protein Comprising the Catalytic Region of Gp63 of Leishmania and LTB against Leishmania donovani