Crystallization and Preliminary X-Ray Diffraction Studies of Two Myotoxic Lys49-Phospholipases A2 Complexed with α-Tocopherol

Juliana I. dos Santos; Agnes A.S. Takeda; Daniela P. Marchi-Salvador; Andreimar M. Soares; Marcos R.M. Fontes

doi:10.2174/0929866054864283

ISSN: 0929-8665
E-ISSN: 1875-5305

Crystallization and Preliminary X-Ray Diffraction Studies of Two Myotoxic Lys49-Phospholipases A2 Complexed with α-Tocopherol
Authors: Juliana I. dos Santos¹, Agnes A.S. Takeda², Daniela P. Marchi-Salvador³, Andreimar M. Soares⁴ and Marcos R.M. Fontes⁵
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¹ Departamento de Fisica e Biofísica, Instituto de Biociencias, UNESP, C. P. 510, CEP 18618-000, Botucatu-SP, Brazil; ² Departamento de Fisica e Biofísica, Instituto de Biociencias, UNESP, C. P. 510, CEP 18618-000, Botucatu-SP, Brazil; ³ Departamento de Fisica e Biofísica, Instituto de Biociencias, UNESP, C. P. 510, CEP 18618-000, Botucatu-SP, Brazil; ⁴ Departamento de Fisica e Biofísica, Instituto de Biociencias, UNESP, C. P. 510, CEP 18618-000, Botucatu-SP, Brazil; ⁵ Departamento de Fisica e Biofísica, Instituto de Biociencias, UNESP, C. P. 510, CEP 18618-000, Botucatu-SP, Brazil;
Source: Protein and Peptide Letters, Volume 12, Issue 8, Nov 2005, p. 819 - 822
DOI: https://doi.org/10.2174/0929866054864283
- Available online: 01 Nov 2005

Abstract

BnSP-7 and BnSP-6, two Lys49-phospholipase A2 isolated from Bothrops neuwiedi pauloensis snake venom, were co-crystallized with α-tocopherol and X-ray diffraction data were collected for both complexes (2.2 and 2.6 Å). A new "alternative" quaternary conformation for these two complexes compared with all other dimeric Lys49-PLA2 has been observed.

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2005-11-01

2025-05-24

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Article Type: Review Article

Keyword(s): bothrops neuwiedi venom; crystallization; lys-phospholipase a; myotoxity; tocopherol; x-ray crystallography

Crystallization and Preliminary X-Ray Diffraction Studies of Two Myotoxic Lys49-Phospholipases A2 Complexed with α-Tocopherol

Abstract

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