Mimicking Reverse Protein Splicing by Three-Segment Tandem Peptide Ligation

James P. Tam; Khee D. Eom

doi:10.2174/0929866054864238

ISSN: 0929-8665
E-ISSN: 1875-5305

Mimicking Reverse Protein Splicing by Three-Segment Tandem Peptide Ligation
Authors: James P. Tam¹ and Khee D. Eom²
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¹ Department of Microbiology and Immunology, Vanderbilt University, MCN A5102A, Nashville, TN 37232. ² Department of Microbiology and Immunology, Vanderbilt University, MCN A5102A, Nashville, TN 37232.
Source: Protein and Peptide Letters, Volume 12, Issue 8, Nov 2005, p. 743 - 749
DOI: https://doi.org/10.2174/0929866054864238
- Available online: 01 Nov 2005

Abstract

Here we report a bi-directional and interchangeable three-segment peptide ligation of N, M, and C-segments, mimicking the reverse process of protein splicing to form, in tandem, a tripartite NMC-peptide using a synthetic intein, a role served by the M-segment with an N-terminal Ser or Thr and a C-terminal thioester.

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2005-11-01

2025-05-25

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Article Type: Review Article

Keyword(s): c-to-n tandem ligation; cys ligation; n-to-c tandem ligation; pro ligation; reverse protein splicing; synthetic intein; three-segment tandem ligation; tripartite nmc-protein

Mimicking Reverse Protein Splicing by Three-Segment Tandem Peptide Ligation

Abstract

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