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- ISSN: 0929-8665
- E-ISSN: 1875-5305
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Expression and Purification of Soluble Non-Fusion Vasostatin in Escherichia coli
- Source: Protein and Peptide Letters, Volume 12, Issue 7, Oct 2005, p. 659 - 661
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- 01 Oct 2005
Abstract
Vasostatin has previously been expressed in fused form or in inclusion body form in Escherichia coli. Here the protein was expressed in soluble non-fusion form in BL21(DE3)pLysS by IPTG induction. The expression level of vasostatin was about 15% of the total cellular protein. The expressed vasostatin was purified and its biological activity was investigated by an endothelial cell proliferation assay.
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