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- ISSN: 0929-8665
- E-ISSN: 1875-5305
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Characterization of the Bacteriophage ΦKMV DNA Ligase
- Source: Protein and Peptide Letters, Volume 12, Issue 7, Oct 2005, p. 645 - 648
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- 01 Oct 2005
Abstract
Gene 17 product (gp17) of the Pseudomonas aeruginosa-infecting bacteriophage phiKMV shows in silico similarity to T7 DNA ligase. In a semi-quantitative activity assay, it is shown that gp17 is a functional, ATP-dependent DNA ligase, in spite of some structural differences related to DNA-binding properties). Enzymatic activity of His6-based purified expression product was optimised (4°C at 24h for sticky end double-stranded DNA fragments) and estimated at 0.5 Weiss U/μg.
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