A Simple Method for Constructing a Tagged Protein

C. Jo; J. Kim; S. A. Jo

doi:10.2174/0929866054696064

ISSN: 0929-8665
E-ISSN: 1875-5305

A Simple Method for Constructing a Tagged Protein
Authors: C. Jo¹, J. Kim² and S. A. Jo³
View Affiliations Hide Affiliations

¹ Department of Biomedical Sciences and Biomedical Brain Research Center, National Institute of Health, 5 Nokbun-dong, Eunpyung-gu, Seoul [122-701], Korea; ² Department of Biomedical Sciences and Biomedical Brain Research Center, National Institute of Health, 5 Nokbun-dong, Eunpyung-gu, Seoul [122-701], Korea; ³ Department of Biomedical Sciences and Biomedical Brain Research Center, National Institute of Health, 5 Nokbun-dong, Eunpyung-gu, Seoul [122-701], Korea;
Source: Protein and Peptide Letters, Volume 12, Issue 7, Oct 2005, p. 631 - 633
DOI: https://doi.org/10.2174/0929866054696064
- Available online: 01 Oct 2005

Abstract

We have developed a simple method for preparing a tagged protein by PCR. With this method any protein sequence can be easily tagged. The techniques include three steps of DNA restriction, ligation and PCR. We could obtain a DNA construct containing SUMO-1 gene with His6 tag sequence with high efficiency by the next day.

Article metrics loading...

/content/journals/ppl/10.2174/0929866054696064

2005-10-01

2025-05-18

From This Site

/content/journals/ppl/10.2174/0929866054696064

dcterms_title,dcterms_subject,pub_keyword

-contentType:Contributor -contentType:Concept -contentType:Institution

10

5

Full text loading...

/content/journals/ppl/10.2174/0929866054696064

Article Type: Review Article

Keyword(s): affinity tag; gene fusion; polymerase chain reaction (pcr); recombinant protein; small ubiquitin-related modifier (sumo)

A Simple Method for Constructing a Tagged Protein

Abstract

From This Site

Most Read This Month

Most Cited Most Cited RSS feed

The LL-37 Antimicrobial Peptide as a Treatment for Systematic Infection of Acinetobacter baumannii in a Mouse Model

FN1 Promotes Thyroid Carcinoma Cell Proliferation and Metastasis by Activating the NF-b Pathway

Wogonin Restrains the Malignant Progression of Lung Cancer Through Modulating MMP1 and PI3K/AKT Signaling Pathway

s A Temporin Derived Peptide Showing Antibacterial and Antibiofilm Activities against Staphylococcus aureus

Upregulation of Connexins in the Rat Hippocampal and Cortical Neurons Following Blockade of NMDA Receptors During Postnatal Development

Long Non-coding RNA LINC00473 Promotes Breast Cancer Progression via miR-424-5p/CCNE1 Pathway

Protease Inhibitors (PIs): Candidate Molecules for Crop Protection Formulations against Necrotrophs

Preparation of IgE Antibody and Distribution of IgE⁺ Secretory Cells in the Palatine Tonsil of Bactrian Camel

Different VH3-binding Protein A Resins Show Comparable VH3-binding Mediated Byproduct Separation Capabilities Despite Having Varied Dynamic Binding Capacities Towards A VH3 Fab

Immunogenicity and Neutralization Potential of Recombinant Chimeric Protein Comprising the Catalytic Region of Gp63 of Leishmania and LTB against Leishmania donovani