Efficient Immobilization of Enzymes on Microchannel Surface Through His-Tag and Application for Microreactor

Masaya Miyazaki; Jun Kaneno; Susumu Yamaori; Takeshi Honda; Maria P. P. Briones; Masato Uehara; Kazunari Arima; Kenichi Kanno; Kenichi Yamashita; Yoshiko Yamaguchi; Hiroyuki Nakamura; Hiroo Yonezawa; Masayuki Fujii; Hideaki Maeda

doi:10.2174/0929866053005854

ISSN: 0929-8665
E-ISSN: 1875-5305

Efficient Immobilization of Enzymes on Microchannel Surface Through His-Tag and Application for Microreactor
Authors: Masaya Miyazaki¹, Jun Kaneno², Susumu Yamaori³, Takeshi Honda⁴, Maria P. P. Briones⁵, Masato Uehara⁶, Kazunari Arima⁷, Kenichi Kanno⁸, Kenichi Yamashita⁹, Yoshiko Yamaguchi¹⁰, Hiroyuki Nakamura¹¹, Hiroo Yonezawa¹², Masayuki Fujii¹³ and Hideaki Maeda¹⁴
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¹ Micro-Space Chemistry Laboratory, National Institute of Advanced Industrial Science and Technology, Japan. ² Micro-Space Chemistry Laboratory, National Institute of Advanced Industrial Science and Technology, Japan. ³ Micro-Space Chemistry Laboratory, National Institute of Advanced Industrial Science and Technology, Japan. ⁴ Micro-Space Chemistry Laboratory, National Institute of Advanced Industrial Science and Technology, Japan. ⁵ Micro-Space Chemistry Laboratory, National Institute of Advanced Industrial Science and Technology, Japan. ⁶ Micro-Space Chemistry Laboratory, National Institute of Advanced Industrial Science and Technology, Japan. ⁷ Micro-Space Chemistry Laboratory, National Institute of Advanced Industrial Science and Technology, Japan. ⁸ Micro-Space Chemistry Laboratory, National Institute of Advanced Industrial Science and Technology, Japan. ⁹ Micro-Space Chemistry Laboratory, National Institute of Advanced Industrial Science and Technology, Japan. ¹⁰ Micro-Space Chemistry Laboratory, National Institute of Advanced Industrial Science and Technology, Japan. ¹¹ Micro-Space Chemistry Laboratory, National Institute of Advanced Industrial Science and Technology, Japan. ¹² Micro-Space Chemistry Laboratory, National Institute of Advanced Industrial Science and Technology, Japan. ¹³ Micro-Space Chemistry Laboratory, National Institute of Advanced Industrial Science and Technology, Japan. ¹⁴ Micro-Space Chemistry Laboratory, National Institute of Advanced Industrial Science and Technology, Japan.
Source: Protein and Peptide Letters, Volume 12, Issue 2, Feb 2005, p. 207 - 210
DOI: https://doi.org/10.2174/0929866053005854
- Available online: 01 Feb 2005

Abstract

We developed a simple immobilisation method for His-tagged enzymes on a microchannel surface. It facilitates immobilisation of protein molecule on microchannel surface through Ni-complex, using crude or purified protein solutions. By this method, we could immobilize proteins on microcapillary constantly. This method might be useful for further development of microreactor with reversibly immobilized enzymes.

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2005-02-01

2025-05-20

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Article Type: Review Article

Keyword(s): enzyme reaction; microreactor; reversible immobilization; surface modification

Efficient Immobilization of Enzymes on Microchannel Surface Through His-Tag and Application for Microreactor

Abstract

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