Crystallization Of The Terminal Oxygenase Component Of Biphenyl Dioxygenase Derived From Rhodococcus Sp. Strain Rha1

Venugopalan Nagarajan; Nobuyuki Sakurai; Miho Kubota; Takamasa Nonaka; Hiroaki Nagumo; Hisashi Takeda; Tomoko Nishizaki; Eiji Masai; Masao Fukuda; the late Yukio Mitsui

doi:10.2174/0929866033478889

ISSN: 0929-8665
E-ISSN: 1875-5305

Crystallization Of The Terminal Oxygenase Component Of Biphenyl Dioxygenase Derived From Rhodococcus Sp. Strain Rha1
Authors: Venugopalan Nagarajan¹, Nobuyuki Sakurai², Miho Kubota³, Takamasa Nonaka⁴, Hiroaki Nagumo⁵, Hisashi Takeda⁶, Tomoko Nishizaki⁷, Eiji Masai⁸, Masao Fukuda⁹ and the late Yukio Mitsui¹⁰
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¹ Division of Protein Engineering and Division of Microbial Engineering, Department of BioEngineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan ² Division of Protein Engineering and Division of Microbial Engineering, Department of BioEngineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan ³ Division of Protein Engineering and Division of Microbial Engineering, Department of BioEngineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan ⁴ Division of Protein Engineering and Division of Microbial Engineering, Department of BioEngineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan ⁵ Division of Protein Engineering and Division of Microbial Engineering, Department of BioEngineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan ⁶ Division of Protein Engineering and Division of Microbial Engineering, Department of BioEngineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan ⁷ Division of Protein Engineering and Division of Microbial Engineering, Department of BioEngineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan ⁸ Division of Protein Engineering and Division of Microbial Engineering, Department of BioEngineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan ⁹ Division of Protein Engineering and Division of Microbial Engineering, Department of BioEngineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan ¹⁰ Division of Protein Engineering and Division of Microbial Engineering, Department of BioEngineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan
Source: Protein and Peptide Letters, Volume 10, Issue 4, Aug 2003, p. 412 - 417
DOI: https://doi.org/10.2174/0929866033478889
- Available online: 01 Aug 2003

Abstract

The terminal oxygenase component of the biphenyl dioxygenase (BphA1A2 complex) was over-expressed with a novel over expression system in recombinant Rhodococcus strain and purified. The purified enzyme has been crystallized by the hanging drop vapor diffusion method and subjected to X-ray diffraction analysis. The crystals belong to the tetragonal system in the space group P4 1 2 1 2 or P4 3 2 1 2 and diffract to better than 2.2Å resolution.

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2003-08-01

2025-04-29

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Article Type: Review Article

Keyword(s): biphenyl dioxygenase; non-heme iron; polychlorinated biphenyl; rieske iron-sulfur center

Crystallization Of The Terminal Oxygenase Component Of Biphenyl Dioxygenase Derived From Rhodococcus Sp. Strain Rha1

Abstract

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