Salting-In Effects offset Mgcl2-Induced Refolding of Nucleoside Diphosphate Kinase

Matsujiro Ishibashi; Tsutomu Arakawa; Masao Tokunaga

doi:10.2174/0929866033478546

ISSN: 0929-8665
E-ISSN: 1875-5305

Salting-In Effects offset Mgcl2-Induced Refolding of Nucleoside Diphosphate Kinase
Authors: Matsujiro Ishibashi¹, Tsutomu Arakawa² and Masao Tokunaga³
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¹ Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan, and Alliance Protein Laboratories, 3957 Corte Cancion, Thousand Oaks, CA 91360, USA. ² Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan, and Alliance Protein Laboratories, 3957 Corte Cancion, Thousand Oaks, CA 91360, USA. ³ Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan, and Alliance Protein Laboratories, 3957 Corte Cancion, Thousand Oaks, CA 91360, USA.
Source: Protein and Peptide Letters, Volume 10, Issue 6, Dec 2003, p. 575 - 580
DOI: https://doi.org/10.2174/0929866033478546
- Available online: 01 Dec 2003

Abstract

Previously we reported that halobacterial nucleoside diphosphate kinase can be refolded in the presence of concentrated trimethylamine N-oxide (TMAO) as well as NaCl, indicating that enhancement of compact structure formation by TMAO is sufficient for folding. Here we showed that the refolding effect of MgCl2 is maximal at 1 M and declines to zero at 2 M, indicating that charge shielding effect of MgCl2 is offset by its salting-in effect.

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2003-12-01

2025-05-03

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Article Type: Review Article

Keyword(s): charge shielding; halophilic; nucleoside diphosphate kinase; refolding; salting-in

Salting-In Effects offset Mgcl2-Induced Refolding of Nucleoside Diphosphate Kinase

Abstract

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