Structural Investigation of Proapoptotic Peptide by Cd and Nmr Spectroscopy

Emy Pulsinelli; Francesca Vasile; Laura Vergani; Silvio Parodi; Claudio Nicolini

doi:10.2174/0929866033478528

ISSN: 0929-8665
E-ISSN: 1875-5305

Structural Investigation of Proapoptotic Peptide by Cd and Nmr Spectroscopy
Authors: Emy Pulsinelli¹, Francesca Vasile², Laura Vergani³, Silvio Parodi⁴ and Claudio Nicolini⁵
View Affiliations Hide Affiliations

¹ Department of Biophysical Sciences and Technologies M., University of Genoa, c.so Europa 30, Genoa, Italy,Laboratory of Experimental Oncology, National Institute for Cancer Research, Largo R. Benzi 10, Genoa, Italy. ² Department of Biophysical Sciences and Technologies M., University of Genoa, c.so Europa 30, Genoa, Italy,Laboratory of Experimental Oncology, National Institute for Cancer Research, Largo R. Benzi 10, Genoa, Italy. ³ Department of Biophysical Sciences and Technologies M., University of Genoa, c.so Europa 30, Genoa, Italy,Laboratory of Experimental Oncology, National Institute for Cancer Research, Largo R. Benzi 10, Genoa, Italy. ⁴ Department of Biophysical Sciences and Technologies M., University of Genoa, c.so Europa 30, Genoa, Italy,Laboratory of Experimental Oncology, National Institute for Cancer Research, Largo R. Benzi 10, Genoa, Italy. ⁵ Department of Biophysical Sciences and Technologies M., University of Genoa, c.so Europa 30, Genoa, Italy,Laboratory of Experimental Oncology, National Institute for Cancer Research, Largo R. Benzi 10, Genoa, Italy.
Source: Protein and Peptide Letters, Volume 10, Issue 6, Dec 2003, p. 541 - 549
DOI: https://doi.org/10.2174/0929866033478528
- Available online: 01 Dec 2003

Abstract

We have performed a systematic investigation of the structural features of the peptides Int (a sequence able to cross cell membranes) and Int-H1(S6A,F8A) (which shows interesting antitumoral properties). After screening in aqueous solution at different ionic strength and pH values, we analyzed the structures of the peptides in different water / trifluoroethanol mixtures by Circular Dichroism and Nuclear Magnetic Resonance techniques.

Article metrics loading...

/content/journals/ppl/10.2174/0929866033478528

2003-12-01

2025-05-06

From This Site

/content/journals/ppl/10.2174/0929866033478528

dcterms_title,dcterms_subject,pub_keyword

-contentType:Contributor -contentType:Concept -contentType:Institution

10

5

Full text loading...

/content/journals/ppl/10.2174/0929866033478528

Article Type: Review Article

Keyword(s): circular dichroism; internalization sequence; molecular dynamics; nmr spectroscopy; proapoptotic sequence; trifluoroethanol

Structural Investigation of Proapoptotic Peptide by Cd and Nmr Spectroscopy

Abstract

From This Site

Most Read This Month

Most Cited Most Cited RSS feed

The LL-37 Antimicrobial Peptide as a Treatment for Systematic Infection of Acinetobacter baumannii in a Mouse Model

FN1 Promotes Thyroid Carcinoma Cell Proliferation and Metastasis by Activating the NF-b Pathway

Wogonin Restrains the Malignant Progression of Lung Cancer Through Modulating MMP1 and PI3K/AKT Signaling Pathway

s A Temporin Derived Peptide Showing Antibacterial and Antibiofilm Activities against Staphylococcus aureus

Upregulation of Connexins in the Rat Hippocampal and Cortical Neurons Following Blockade of NMDA Receptors During Postnatal Development

Long Non-coding RNA LINC00473 Promotes Breast Cancer Progression via miR-424-5p/CCNE1 Pathway

Protease Inhibitors (PIs): Candidate Molecules for Crop Protection Formulations against Necrotrophs

Preparation of IgE Antibody and Distribution of IgE⁺ Secretory Cells in the Palatine Tonsil of Bactrian Camel

Different VH3-binding Protein A Resins Show Comparable VH3-binding Mediated Byproduct Separation Capabilities Despite Having Varied Dynamic Binding Capacities Towards A VH3 Fab

Immunogenicity and Neutralization Potential of Recombinant Chimeric Protein Comprising the Catalytic Region of Gp63 of Leishmania and LTB against Leishmania donovani