A Direct Continuous pH-Spectrophotometric Assay for Arginine Kinase Activity

A direct and continuous spectrophotometric method was developed for determining arginine kinase (phosphoarginine synthesis) activity. Protons are produced during the phosphoarginine synthesis course, so adding the complex acid-base indicator to this solution and monitoring the decrease of absorbance of the solution at 575 nm will follow the arginine kinase activity. For this condition, one activity unit of arginine kinase was defined as 1 μmol H+ produced in 1 minute in the enzymatic reaction catalyzed by arginine kinase.