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image of The Constituents Analysis and the Anti-inflammatory Activity of the Chinese Medicine: Kanglao Concoction

Abstract

Introduction

The Chinese medicine, Kanglao concoction has been used clinically to treat tuberculosis. However, its constituents and anti-inflammatory activity have not been fully understood.

Method

In this study, HPLC-MS2 has been employed to determine the main constituents in the concoction. The anti-inflammatory activity has been investigated by ELISA and Western Blot techniques. The MS analysis revealed that tigogenin and afzelin were two main constituents in Kanglao concoction.

Result

Moreover, the result of ELISA experiments showed that Kanglao concoction significantly reduced the expression of TNF-α and IL-1β at 5 mg/mL and 10 mg/mL to inhibit inflammation.

Conclusion

The expression of Notch pathway-related proteins in A549 cells increased under the stimulation of , and decreased dose-dependently upon the treatment of Kanglao concoction, which was deduced to inhibit Notch pathway expression to impose anti-inflammatory activity.

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2024-10-29
2025-05-10

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The Constituents Analysis and the Anti-inflammatory Activity of the Chinese Medicine: Kanglao Concoction
Bentham Science Publishers ; https://doi.org/10.2174/0122103155337218241021045316
/content/journals/npj/10.2174/0122103155337218241021045316
/content/journals/npj/10.2174/0122103155337218241021045316
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  • Article Type:     Research Article
Keywords: A549 cells ; kanglao concoction ; Anti-inflammatory ; notch pathway ; MS analysis

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