Current Proteomics - Volume 18, Issue 2, 2021

The current global pandemic outbreak of a novel type of coronavirus termed by the World Health Organization as COVID-19 became a grave concern and worry to human health and the world economy. Intense research efforts are now underway worldwide to combat and prevent the spread of this deadly disease. This zoonotic virus, a native to bat population, is most likely transmitted to a human via a host reservoir. D Read More

Background: In our previous study, six cultivars of Mungo (Vigna radiata) were exposed to 100-250 mM NaCl and studied for changes in growth and biomass. Among them, AEM-96 cultivar of the Mung bean [Vigna radiata (L.) Wilczek cv.] was found to tolerate NaCl stress at 250 mM. Objective: The soluble proteome of salt-tolerant mungo cultivar (AEM-96) was compared to the proteome of control mungo to investigate the possi Read More

Background: Leptospirosis is a prevalent zoonotic disease caused by Leptospira interrogans bacterium. Despite the importance of this disease, traditional strategies including attenuated and inactivated vaccines have not been able to prevent leptospirosis. Objective: Hence, this study was designed to develop a novel poly-epitope fusion protein vaccine against Leptospirosis. Methods: To do so, the best epitopes of OmpA, Lip Read More

Background: Excluding humans, the peroxisomal uricase is responsible for the catabolism of uric acid into allantoin in many species like microorganisms, plants, and invertebrates. Particularly in humans, the synthesis and excretion of uric acid are naturally balanced. When the uric acid concentration crosses 7 mg/dl, it results in conditions such as hyperuricemia and gout. Uricase is one of the potential sources for th Read More

Background: Diabetic Retinopathy (DR), one of the major microvascular complications commonly occurring in diabetic patients, can be classified into Proliferative Diabetic Retinopathy (PDR) and Non-Proliferative Diabetic Retinopathy (NPDR). Currently, available therapies are only targeted for later stages of the disease in which some pathologic changes may be irreversible. Thus, there is a need to develop new treatment optio Read More

Background: The rate of Human Immunodeficiency Virus type 1 (HIV-1) infection in Iran has increased dramatically in the last few years. Objective: The aim of this study was to investigate the HIV subtype amongst all Iranian HIV sequences, using 8 websites. Methods: In this study, 637 sequences of polymerase, and gag genes of HIV-1 were obtained from NCBI. HIV-1 subtyping was done, using 8 reliable software. Result Read More

Aims: The importance of cathelicidin-1 as an indicator of the severity of mammary infection in ewes. Background: Mastitis is an important disease of sheep, affecting their health and welfare. Objective: The association of the presence of cathelicidin-1 in milk samples from ewes with mastitis with the severity of the infection. Methods: Ewes were intramammarily inoculated with Mannheimia haemolytica or Staphylococcus chro Read More

Background: Lipid homeostasis and gut flora can be related to many metabolic diseases, especially autism. Lipid metabolism in the brain can control neuronal structure and function and can also take part in signal transduction pathways to control metabolism in peripheral tissues, especially in the liver. Impaired phospholipid metabolism promotes oxidative stress and neuroinflammation and is, therefore, directly rel Read More

Background: Human Papillomavirus (HPV) is responsible for substantial morbidity and mortality worldwide. We predicted immunogenic promiscuous monovalent and polyvalent T-cell epitopes from the polyprotein of the Human Papillomavirus (HPV) using a range of bioinformatics tools and servers. Methods: We used immunoinformatics and reverse vaccinology-based approaches to design prophylactic peptides by antigenici Read More

Background: Oxaliplatin(Oxa) is a major chemotherapy drug for colorectal cancer. However, drug resistance is a major cause of treatment failure for late-stage colorectal cancer. Therefore, it is necessary to explore the mechanism of resistance to oxaliplatin in HCT116 colorectal cancer cells. Objective: Therefore, this study explored the mechanisms of HCT116 cells resistance to oxaliplatin by combining the results of proteomi Read More

Background: Cancers result due to the dysregulation of gene expression. They can be identified on the basis of driver mutations and genetic signatures. Proteins are macromolecules that regulate the structure and function of body organs. Missense somatic mutations play a critical role in the development of cancer by altering the underlying properties of corresponding proteins. The extent to which the chemical properties Read More

Background: Although the food quality of soy protein is known to be as good as that of animal proteins, some soybean proteins are not susceptible to digestion and remain undigested in the intestine. We hypothesized that digestion-resistant soy proteins might interact with the intestinal membrane, microbes, and metabolites, and change the intestinal physiology or the profile of the gut microbiome. Objective: To identify t Read More

Background: To investigate the interactions between RNA and proteins is essential to understand how these macromolecule complexes exert their functions. RNA pull-down is a classic technique to enrich RNA binding proteins, however, a large number of non-specific binding proteins may be enriched during sample preparation, interfering with the downstream mass spectrometric analyses and also causing false positives. Obj Read More

Background: Symptomatic intracranial atherosclerotic Disease (sICAD) is associated with the risk of recurrence of cerebral ischemic events in 4-19% of stroke patients annually. Previous studies indicate elevated high-sensitivity C-Reactive Protein (hs-CRP) and lipoprotein-associated phospholipase A2 (Lp-PLA2) to be associated with risk of recurrence. Objective: This prospective, observational study investigated serum Read More

Background: Helicobacter pylori infection and its treatment still remain a challenge for human health worldwide. A variety of antibiotics and combination therapies are currently used to treat H. pylori induced ulcers and carcinoma; however, no effective treatment is available to eliminate the pathogen from the body. Additionally, antibiotic resistance is also one of the main reasons for prolonged and persistent infection. Ai Read More

Aims: Exploring molecular mechanism of abiotic stress tolerance in plants is needed to overcome the deterioration of yield and quality of crop plants to meet the food security challenges of the growing population. Background: MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate target gene expression for modulating plant growth, development, and response to different stresses. Agave belonging to CAM Read More