Current Proteomics - Volume 14, Issue 4, 2017

Background: At the cellular level, normal chromosome segregation is ensured by the intrinsic mechanics of mitosis and the proper functioning of the error-checking spindle assembly checkpoint (SAC). Protein Mad1 (the mitotic arrest-deficient), an important SAC component, plays a crucial role in avoiding cellular aneupoidy, a state leading to genetic diseases such as cancer or bipolar disorder. Objective: To clarify the role of ane Read More

Background: RNA binding Proteins (RBPs) influence the structure and function of RNA and play a very important role in many biological processes like transcription, translation, cell cycle and gene silencing mechanisms. Objective: Structural studies of spliceosome machinery and proteins involved in gene silencing mechanisms are of prime importance as it is poorly understood in Neurospora crassa. In view of this, the pres Read More

Background: Methamphetamine is a well-known psychostimulant, which exerts its influence on the central nervous system. To identify the changes caused by this drug at the molecular level, several proteomic studies were performed, their results were published in available literature. Objective: We summed up the results of available proteomic data on the topic of methamphetamine influence on central nervous system publishe Read More

Background: The ribosome binding site (RBS) containing Shine-Dalgarno (SD) sequence in prokaryotes plays an important role in identification of the translation initiation site within mRNA by ribosome. Objective: Our study aimed to improve the target protein production under translational level in prokaryotes by engineering of RBSs. Method: Two RBSs were designed in Streptomyces coelicolor M145, Sco-RBS* with an SD se Read More

Background: Water buffalo (Bubalus bubalus) are domesticated animals of economic importance in many Asian countries, whereas the basic helix-loop-helix (bHLH) transcription factors (TFs) have been analyzed in the genomes of many eukaryote organisms. However, the bovine bHLH TFs have not been characterized and their specific functions remain unknown. Objective: To better characterize and functionally understand Read More

Background: Halophiles, which thrive under saline/hypersaline habitats and retain their structural and functional integrity under high salt conditions, present an interesting model system for understanding their global proteome under different salinity. Objective: The present work compares the intracellular proteome profiles of two moderately halophilic Gram positive Bacillus sp. EMB9 and Gram negative Marinobacter sp. EMB5 i Read More

Background: Solubility of recombinant therapeutic enzymes is one of the major purpose their production in bacterial host The factors such as metabolic system, a cellular stress response of the bacterial host and formation of inclusion bodies cause undesirable expression results. One method to overcome this problem is secretion of recombinant protein into the extracellular growth medium by selection of proper signa Read More