Current Proteomics - Volume 14, Issue 3, 2017

Background: Polyploid breeding is a powerful approach for Populus genetic improvement because polyploid trees have valuable characteristics, including better timber quality and a higher degree of stress resistance compared with their full-sib diploids. However, the genetic mechanism underlying this phenomenon remains unknown. Objective: To better understand the proteomic changes between Populus allotriploids an Read More

Background: Pullulanase is broadly used in saccharification process for glucose, maltose, maltotriose, and fructose production. The in silico investigation can be a useful approach to screen an enzyme with suitable characteristics for application in industry. Objective: The aim of this study was an in silico characterization and functional analysis of pullulanases from different Bacillus species. Method: A total number of 38 ami Read More

Background: Meta-prediction seeks to combine the strengths of multiple predicting programs with the hope of improving the predicting performance surpassing that of all existing predictors. As increasing numbers of predicting programs have been developed in this field, there is an urgent need for effective meta-prediction strategies. Objective: In this paper, we apply meta-prediction for the identification of protein-p Read More

Background: The fibroblast growth factor (FGF) family is essential to tissue development and stem cell differentiation; therefore, there is increasing interest in the clinical application of FGFs, resulting in increasing demand for active recombinant human FGFs (rhFGFs). Objective: To improve the production of rhFGF18, we determined the optimal culture conditions required to maximize rhFGF18 protein expression E. coli. Method: Read More

Background: The relevance of bioinformatics is being realized in the era of genomics, assisting in genome-wide identification and characterization of putative gene families of different enzymes for diverse industrial applications. Therefore, in the present manuscript, Mn peroxidases protein sequences from different fungal sources were subjected to bioinformatics assessment for homology search, multiple sequence alignme Read More

Objectives: Hypertension exerts a chronic tensile stretch on the endothelial lining of the inner layer of blood vessels and is thought to cause cellular injury leading to numerous pathologies. Studies have indicated that tensile stretch could alter the phenotype and cellular functions of the cell, depending on the stretch magnitude applied. However, the molecular mechanisms of the cellular injury under these conditions remain u Read More

Objectives: Cyclophilin D (CypD) is a chief regulatory protein of the necroptosis pathway involved in various neurological disorders, and ablation/inhibition of this protein confers neuroprotection. Current in silico drug design strategies employ multiple structures of a protein target since they enable the identification of diverse inhibitor molecules. However, structure-based drug design against a protein target becom Read More

Background: Hepatic cirrhosis is associated with a variety of hemodynamic and hydrodynamic abnormalities, including renal dysfunction with salt and water retention leading to ascites and dilutional hyponatremia. Objective: In the present study, a proteomic analysis of the kidney of rats subjected to bile duct ligation (BDL) has been carried out in order to identify the proteins altered under this condition. Method: The expe Read More

Objectives: The main goal of this study was to decipher the role of CSA homologues in the DNA damage repair machinery of Arabidopsis thaliana. Method: Bioinformatics tools were used in this work to analyse DNA Damage-Binding proteins; MSA, Phylogenetic tree construction, 3-D structure prediction, domain analysis, subcellular localization prediction, interactome analysis and docking sites. Result: The pairwise alignment fr Read More