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Volume 18, Issue 2
  • ISSN: 2772-2708
  • E-ISSN: 2772-2716
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Abstract

At present, no proven effective treatment is available for Lung Ischemia-reperfusion Injury (LIRI). Natural compounds offer promising prospects for developing new drugs to address various diseases. This study sought to explore the potential of Rebaudioside B (Reb B) as a treatment compound for LIRI, both and .

This study involved utilizing the human pulmonary alveolar cell line A549, consisting of epithelial type II cells, subjected to Oxygen-glucose Deprivation/recovery (OGD/R) for high-throughput cell viability screening. The aim was to identify the most promising candidate compounds. Additionally, an rat model of lung ischemia-reperfusion was employed to evaluate the potential protective effects of Reb B.

Through high-throughput screening, Reb B emerged as the most promising natural compound among those tested. In the A549 OGD/R models, Reb B exhibited a capacity to enhance cell viability by mitigating apoptosis. In the LIRI model, pre-treatment with Reb B notably decreased apoptotic cells, perivascular edema, and neutrophil infiltration within lung tissues. Furthermore, Reb B demonstrated its ability to attenuate lung inflammation associated with LIRI primarily by elevating IL-10 levels while reducing levels of IL-6, IL-8, and TNF-α.

The comprehensive outcomes strongly suggest Reb B's potential as a protective agent against LIRI. This effect is attributed to its inhibition of the mitochondrial apoptotic pathway and its ability to mitigate the inflammatory response.

© 2024 Bentham Science Publishers
© 2024 The Author(s). Published by Bentham Science Publisher. This is an open access article published under CC BY 4.0 https://creativecommons.org/licenses/by/4.0/legalcode
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2024-09-01
2025-01-20

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/content/journals/raiad/10.2174/0127722708295154240327035857
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Rebaudioside B Attenuates Lung Ischemia-reperfusion Injury Associated Apoptosis and Inflammation
Rec Adv Inflamm Allergy Drug Discov 18, 156 (2024); https://doi.org/10.2174/0127722708295154240327035857
/content/journals/raiad/10.2174/0127722708295154240327035857
/content/journals/raiad/10.2174/0127722708295154240327035857
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  • Article Type:     Research Article
Keyword(s): A549; apoptosis; high-throughput screening; lung ischemia-reperfusion injury; oxygen-glucose deprivation/recovery; Rebaudioside B

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