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image of Recombinant Production of Ib-AMP4 and Oncorhyncin II Antimicrobial Peptides and Antimicrobial Synergistic Assessment on the Treatment of Staphylococcus aureus Under in vitro Condition

Abstract

Background

Methicillin-resistant (MRSA) is a significant and prevalent pathogen that poses a major challenge in healthcare environments. In light of the growing threat posed by multidrug-resistant organisms like MRSA, there is an urgent need for alternative therapeutic strategies. One promising avenue of research involves the use of antimicrobial peptides (AMPs). These naturally occurring molecules, which are part of the innate immune response in many organisms, have garnered attention for their ability to combat a wide range of pathogens.

Objectives

This study aimed to produce recombinant versions of Ib-AMP and Oncorhyncin II and to evaluate their combined effects against MRSA (NCTC10442).

Methods

DE] served as the expression host for the synthesized variants of the Ib-AMP4 and Oncorhyncin II genes. The antimicrobial efficacy of these peptides against MRSA (NCTC1042] was evaluated using a comprehensive methodology that encompassed the determination of the minimum inhibitory concentration (MIC), the performance of time-kill assays, and the analysis of growth kinetics.

Results

The individual antimicrobial activities of Ib-AMP and Oncorhyncin II were assessed, revealing minimum inhibitory concentrations (MICs) of 27.75 µg/mL and 40.125 µg/mL against (MRSA) (NCTC10442), respectively. The application of a checkerboard assay to evaluate the combination of these antimicrobial peptides (AMPs) demonstrated a synergistic interaction, which was further validated through time-kill and growth kinetic studies. When administered at double the MIC, a significant reduction in the log CFU/mL of MRSA (NCTC 10442) was observed, underscoring the synergistic bacteriostatic effect mediated by the fractional inhibitory concentration (FIC) index of the two peptides.

Conclusion

Antimicrobial peptides (AMPs) have attracted significant interest owing to the growing intricacy of microbial infections. They constitute a promising category of novel antibiotics that warrant further investigation for the treatment of infections and the enhancement of wound healing. Although certain AMPs can operate autonomously, others may necessitate a synergistic approach alongside conventional antibiotics. Studies examining the combined efficacy of Oncorhyncin II and Ib-AMP against MRSA have revealed their effectiveness.

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2024-11-26
2025-01-19

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Recombinant Production of Ib-AMP4 and Oncorhyncin II Antimicrobial Peptides and Antimicrobial Synergistic Assessment on the Treatment of Staphylococcus aureus Under in vitro Condition
Bentham Science Publishers ; https://doi.org/10.2174/0109298665327474241112093601
/content/journals/ppl/10.2174/0109298665327474241112093601
/content/journals/ppl/10.2174/0109298665327474241112093601
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  • Article Type:     Research Article
Keywords: Oncorhyncin II ; antimicrobial peptide ; Staphylococcus aureus ; Ib-AMP4

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