Current Pharmaceutical Analysis - Volume 9, Issue 4, 2013

Analysis of n3 fatty acids in serum samples has clinical applications in supplementation trials, but the analysis can be challenging due to low levels, stability issues and intra-individual variation. This study presents the single laboratory validation of a gas chromatographic-mass spectral (GC-MS) assay for analysis of fatty acid methyl esters (FAME) using sensitive single ion monitoring and provides data on fatty acid stability under Read More

This work aims to develop and validate a simple methodology to quantify the most used antiretroviral, zidovudine (AZT), in rat plasma for preclinical studies. Some assays have been previously reported to quantify AZT in plasma; however, the majority of these methods uses complicated extraction methods. This method uses only 100 μL plasma samples, which were precipitated with 100 μL acidified acetonitri Read More

The preparation of a parenteral solution for oral use leads the clinician to question the stability of drugs when used in conjunction with flavored oral suspending vehicles. Therefore, the purpose of this study was the evaluation of the stability of midazolam, frequently used as an oral premedication in children, in extemporaneously prepared pediatric syrup, using micellar electrokinetic capillary chromatography (MEKC). The optimizat Read More

Measurement uncertainty provides indication of the quality of the results and its usefulness for compliance or non-compliance decisions. On the other hand, pharmaceutical equivalence is an important step in confirmation of similarity and interchangeability of pharmaceutical products. The aim of this work was to establish a procedure to estimate the measurement uncertainty for metronidazole quantification by high perfor Read More

In this article we present an update on the applications of chemiluminescence coupled with flow injection analysis for the determination of pharmaceuticals. Several reported applications of flow and sequential injection chemiluminescence (FI-CL/SI-CL) in pharmaceuticals concern greatly on quality control, investigation of dissolution of particular formulations or process control of production of pharmaceuticals and Read More

In this research, we aimed to develop a new triadimefon microemulsion according to the pseudoternary phase diagrams which could greatly decrease the usage of organic solvents while improve stability and antifungal activity of triadimefon preparation. The microemulsion was successfully prepared using toluene as solvent, emulsifier HSH as surfactant, and methanol as co-surfactant. And its optimal formulation Read More

The aim of this study is optimization of experimental factors in a UV-visible spectrophotometric method for simultaneous determination of dorzolamide hydrochloride and timolol maleate in eye drops. For this purpose, partial least squares-1 method was developed for resolving overlapped UV-visible spectra of these compounds. Principal component number, wavelength range (number of wavelengths) and data Read More