Current Pharmaceutical Analysis - Volume 9, Issue 1, 2013

Simvastatin, a lipid-lowering agent of the statins class, is widely used in the treatment of hypercholesterolemia and in the prevention of cardiovascular diseases. Currently, simvastatin is available in the global market in both tablet and capsule forms. No official method has been reported for the assaying or dissolution testing of simvastatin capsules. Dissolution specifications were established to evaluate in vitro re Read More

The Medicinal Plant of Corrientes, Argentina, elaborates, distributes and provides free 500 mg cephalexin tablets in hospitals and health care centers. HPLC-UV is the reference method to quantify this antibiotic. The spectrophotometric ninhydrin method was applied for cephalexin quantification and analytical parameters for its validation were determined. The linearity of this method was in the range 0.04-0.20 mg/ Read More

Amikacin (AMK) is a semi-synthetic aminoglycoside antibiotic used under the sulfate salt form and manufactured under parenteral formulations. The determination of the sulfate concentration is important to confirm the completeness of salt formation. Continuing the approach followed by some authors in FTIR spectroscopy, the principle of using analyses of aqueous samples in the mid-IR region, by transmission, was adopt Read More

S-nitrosoglutathione (GSNO) is one of low molecular weight S-nitrosothiols occuring in humans. Nowadays, it is widely used as a nitric oxide donor for in vitro, ex vivo and in vivo experiments related to the investigation of its pathophysiological role as well as in clinical trials, aimed at its potential therapeutic use. Despite numerous reports on this physiological molecule, its quality control does not match the criteria required by co Read More

This work determines the total protein concentration present in microsome fractions obtained by two distinct extraction procedures: ultracentrifugation and calcium aggregation. Additionally, the batch to batch variability within the extraction procedures used and the microsome stability and activity at -80°C during 90 days were determined using 7- ethoxyresorufin as a marker substract. An HPLC method to deter Read More

A novel 96-microwell-based spectrophotometric assay has been developed and validated for the determination of rosuvastatin calcium (ROS-Ca) in tablets. The formation of a colored charge-transfer (CT) complex between rosuvastatin calcium (ROS-Ca) as n-electron donor and 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) as a π-electron acceptor was investigated, for the first time. The spectral characteristics of the CT co Read More

An accurate, precise, simple and selective stability-indicating gradient reverse phase ultra performance liquid chromatographic method has been developed and validated for the quantitative determination of rupatadine and montelukast in pharmaceutical formulation in presence of degradation products. The chromatographic separation was performed on Acquity BEH C8 column (100 mm x 2.1 mm I.D., 1.7 μm) by u Read More

This present review submitted high-performance liquid chromatography (HPLC) methods for determining the presence of meloxicam used in clinical treatment within various fields of application, including biological samples and pharmaceutical formulations, between 1990 and 2011. Meloxicam is a cyclooxygenase-2 selective nonsteroidal anti-inflammatory drug (NSAIDs), which is the group most often used in human Read More

The aim of this work is to develop and validate a microbiological assay for ceftriaxone with a view to the employment of this method to assess photo-stability in commercial products. The experimental conditions included: (1) ceftriaxone standard solution in concentrations of 2.6 μg/mL, 3.2 μg/mL, 4.0 μg/mL, 5.0 μg/mL and 6.3 μg/mL, using phosphate buffer pH 6.0 as diluent, (2) Petri dishes prepared by adding 21-mL of anti Read More

The quality of clopidogrel (CLP) and acetylsalicylic acid (ASA) combination dosage forms was evaluated for their content, impurities and drug release. An enantiospecific (chiral) method for the quantification of the R-enantiomer of CLP and an achiral method for the determination of the content of CLP and ASA and their impurities were used. The following stationary phases were used: a CHIRALCEL® OJ column (250 mm Read More

A simple, rapid and sensitive RP-HPLC method was developed and validated for the quantification of bromocriptine mesylate in bulk drug and tablet formulation. The separation was achieved on a Zorbax Eclipse XDB-C18 column (150 mm x 4.6 mm i.d.). The mobile phase consisted of methanol and 20 mM sodium acetate, pH 5 (70:30, v/v) at a flow rate of 1.5 mL min-1 and detection was performed at 300 nm using photodiode a Read More

Ketoconazole (1-[4-(4-{[(2R,4S)-2-(2,4-dichlorophenyl)-2-(1H-imidazol-1-ylmethyl)-1,3-dioxolan-4-yl]-methoxy} phenyl)piperazin-1-yl]ethan-1-one) in substantia was irradiated to doses between 25 kGy (standard sterilizing dose) and 800 kGy, using high energy electrons from an electron accelerator. The irradiated and non-irradiated samples were then examined using a variety of spectroscopic (UV, MS, NMR, EPR), chrom Read More

A flow-based system with solenoid micro-pumps is proposed for fast simvastatin (SMT) determination in pharmaceutical preparations. The method is based on the inhibition of methyl orange bromination in the presence of the analyte with spectrophotometric detection. A linear response was observed between 5.0 and 35 mg L-1 SMT with a detection limit of 1.2 mg L-1 (99.7% confidence level). The coefficient of variation (n = Read More

Calixarenes (CAs), with their unique three-dimensional surface, are one of the best known host molecules along with cyclodextrins, and crown ethers. However, these compounds have been less studied compared to cyclodextrins. The present work is devoted to the evaluation of the ability of eleven different calixarenes to form host-guest complexes with eleven pharmaceutical relevant substances (chiral active pharmaceut Read More