Current Pharmaceutical Analysis - Volume 9, Issue 2, 2013

The extrapyramidal side effect parameters of typical and atypical antypsychotics were correlated with affinity chromatographic data determined on the melanin-based column. The chromatographic study was performed according to the hypothesis that extrapyramidal symptoms (EPS) as side effects of the use of antipsychotic drugs at clinically effective doses are correlated to the affinity of these drugs to neuromelanin. For tha Read More

Tablet splitting has been a practice widely used. The aim of this work is to investigate the influence of splitting on content uniformity of 25 mg captopril tablets, a common dosage form. Split tablets were obtained by using the hands, a knife or a tablet-splitter. Captopril reference product and two different brands codified as X and Y were used in this study. The method used for quantification was that described in The Unite Read More

Column switching LC/MS methods were developed for rapid identification of impurities in cefdinir. Based on the mechanism by which cephalosporins are degraded, stress tests were designed and performed. It was found that eight main impurities were degradation products and five impurities originated from the synthesis process. Cefdinir bulk material was eluted as gradient on a C18 column, with 0.25% tetram Read More

A specific ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established and validated for the simultaneous quantification of paclitaxel prodrug (PP) and paclitaxel (PT) in human plasma. PP, PT and an internal standard 13C6-labelled PT (13C6-PT) were extracted from plasma by tert-butyl methyl ether and separated on an ACQUITY UPLC BEH C18 column (50 mm × 2.1 mm, 1.7 Read More

The purpose of this study was to develop a sensitive and rapid method for the determination of venlafaxine in human saliva. Blank saliva samples were obtained from healthy volunteers. Their extraction was carried out on SPE columns. Cartridges of the columns were washed with water and the adsorbate was eluted with methanol. The eluate was evaporated and the residue was dissolved in a mixture of acetonitrile and wate Read More

The aim of this work was to develop a microbiological assay of vancomycin employing microplates and kinetic reading mode, and to validate the developed method by assessing of specificity and selectivity, linearity, linear range, accuracy and precision parameters. Established conditions comprised a vancomycin standard-curve in concentrations from 0.6 to 1.0 μg mL-1, and soybean-casein broth inoculated with 5% of Ba Read More

Two different strategies were used to encapsulate oleanolic acid into nanoparticles, including nanoprecipitation and liposome technique. In nanoprecipitation method, the detailed formulations were investigated by varing the parameters of total good solvent/poor solvent volume ratio, surfactant mass ratio. The effects of different physical situations were considered in the experiment. The formulation which exhibits the Read More

Resolution of ternary and quaternary mixtures of paracetamol (PAR), desxtromethorphan (DEX), phenylephrine (PHE), and chlorpheniramine (CHL) was successfully achieved with minimum sample pre-treatment and without analyte separation using a rapid method based on partial least squares (PLS) and UV spectral data. Multivariate PLS calibration was performed using 35 ternary and quaternary mixtures and the model was e Read More

A simple, sensitive and rapid isocratic reversed phase (RP) liquid chromatographic (LC) method was developed for the estimation of milnacipran in bulk and its formulations. Separations were carried out using the mobile phase consisting of 25mM phosphate buffer, acetonitrile and methanol on LiChrospher® 100 RP-18 analytical column at a flow rate of 1.0 mL/min and detection wavelength of 220 nm. The method developed as Read More

Quantification of pegylated drugs in biological matrices is often complex and challenging. This paper describes a sensitive HPLC method with fluorescence detection for the analysis of pegylated resveratrol, resveratrol and its metabolites. Solvent mediated fluorescence enhancement and quenching effects were explored to develop a highly sensitive HPLC method for analysis of resveratrol-PEG conjugates. The effect of sol Read More

The antioxidant capacity of capsules containing blueberry based products which are included among the group of integrators owing to their antioxidant capacity and produced by various drug firms was investigated. The results of the investigation are compared to rank these products in order to their antioxidant capacity. In order to measure antioxidant capacity, our laboratory has recently developed a special electroche Read More

A comprehensive approach using multiple qualitative and quantitative methods coupled with multivariate statistical analysis was developed for the quality evaluation of Arnebiae Radix currently marketed in China. A simple, rapid and accurate liquid chromatographic method by optimization of the current ones was established for determination of β, β'-dimethylacrylalkannin. Moreover, hierarchical clustering analysis (HCA) and Read More

Although there are many different methods for measuring the carbon monoxide and carbon dioxide content under biological conditions, not all of them are appropriate for detecting the very low concentrations. This review discusses the principles of the most commonly used methods for the detection and quantitative determination of these species, as well as the advantages and limitations of the methods described. The cons Read More