Current Pharmaceutical Analysis - Volume 7, Issue 2, 2011

This paper describes a stripping method for the determination of oseltamivir (Tamiflu) at the submicromolar concentration level. This method is based on controlled adsorptive accumulation of oseltamivir at the thin-film mercury electrode followed by a linear cyclic scan voltammetry measurement of the surface species. Optimum experimental conditions consist of a NaOH solution (pH 7-8) as supporting electrolyte, a Read More

Nadolol and propranolol are beta-blockers used to prevent the onset of migraines. Two simple and rapid High Performance Liquid Chromatographic (HPLC) methods with ultraviolet detection were evaluated in order to establish their efficacy for detecting nadolol and propranolol hydrochloride in samples obtained from in vitro transdermal absorption studies. Both methods were validated for specificity, linearity, precision, accurac Read More

The electrochemical behaviors of eugenol [1-hydroxy-2-methoxyl-4-allylbenzene] on the gold and platinum electrodes were investigated in a Britton-Robinson buffer (pH 1.60-10.44), acetate buffer, phosphate buffer solutions (pH 2.11 and 3.67) and methanol or acetonitrile containing various supporting electrolytes. The detection cell consisted of a gold wire electrochemical signal obtained with a supporting electrolyte Read More

A rapid and sensitive liquid chromatography/tandem mass spectrometry (LC-MS/MS) method was developed and validated for the determination of asiaticoside in beagle dog plasma. Plasma samples were extracted by protein precipitation with methanol. The chromatographic separation was performed on a C18 column with the isocratic mobile phase comprising of 78% methanol in water and 10 mM ammonium acetate buffe Read More

A static headspace gas chromatographic (sHS-GC) method for quantitative determination of residual organic solvents in eprosartan mesylate drug substance is developed. SE-30 capillary column (30 m x 0.25 mm i.d., 0.5 μm film thickness) and FID detector are adopted. Response surface methodology (RSM) has been successfully applied to obtain the optimum headspace conditions: the equilibrium temperature is set at 80 Read More

Natural matrices comprise a continuous resource of immeasurable biological activities and chemical entities. The diversity of marine ecosystems has provided a unique source of chemical compounds with potential bioactivities that could lead to new drug candidates. In fact, as many marine-living organisms are soft bodied and/or sessile, over evolutionary time marine eukaryotes have developed an array of metabolites and s Read More

Curcumin is the active ingredient of turmeric which is widely used as a yellow spice and food additive in Asia, India and western world. It is a complex natural extraction with various biological target points and different cellular activities including anti-inflammation, antioxidant, decreasing blood lipid, regulating human immunity function and antitumor effect. Recently, its anti-tumor mechanisms have been thoroughly investi Read More

Background: Tabernaemontana divaricata (L.) R. Br. (Apocynaceae) is an evergreen usually glabrous tree found throughout the India, Australia and Polynesia. Plant roots are chewed for the relief of tooth rubbed into a thin paste and administered as vermicide, also used with lime juice to clear opacity of cornea, juice of leaves used as cooling application for wounds to prevent inflammations. The tree species has special interest Read More

Herbal medicine is becoming more and more popular and has been widely used in the world. However, the quality control is a major issue for different types of herbal products. In this study, a combination of high performance liquid chromatography (HPLC) and gas chromatography (GC) analytical methods was successfully employed for the pharmaceutical analysis and quality control of herbal medicine. Five different bran Read More