Current Pharmaceutical Analysis - Volume 7, Issue 3, 2011

Hypoglycemic drugs are popular for the treatment of Type-2 diabetes. In general, they are administered in oral doses daily and are widely biotransformed in the body. A large number of analytical methods based on chromatographic and electrophoretic techniques for the quantification of hypoglycemic drugs and their metabolites in biological matrices have been described in literature and have been used to support pre Read More

Hepcidin is a small protein involved in iron metabolism that also exhibits antibacterial and antifungal activities. Since its discovery in 2000, hepcidin has been extensively studied in many research centers. Usage of natural and synthetic hepcidin inhibitors can be applied as a powerful therapeutic tool that targets multiple types of anemia. In addition, the protein itself has a potential to placate increased absorption of iron in patients Read More

High performance liquid chromatographic and 1st derivative spectrophotometric methods for simultaneous determination of chlorhexidine gluconate (CG) and benzydamine hydrochloride (BH) in gargle and spray preparations used for the treatment of dental illnesses, mouth and faucet infections and providing oral hygiene were developed and validated. CG and BH responses were respectively measured at 271 nm and 3 Read More

A stability-indicating high-performance liquid chromatographic method was developed and validated for the determination of orbifloxacin in tablet dosage forms. Reversed-phase chromatography was performed on a Symmetry Shield RP-18 Waters®, 250mm×4.6mm i.d., 5 μm particle size column with 5% acetic acid:methanol (80:20, v/v) as mobile phase at a flow rate of 0.7 ml/min. PDA detection was performed at 290 nm Read More

In this present research work, a new stability indicating assay method was developed for the estimation of trandolapril and its degraded products by isocratic reversed phase chromatographic technique using ultra performance liquid chromatography. The Study involves a comprehensive stress testing of trandolapril which was carried out according to ICH guideline Q1A (R2). The drug was subjected to acid (0.1M HCl), neutral (wa Read More

Ocular pharmacokinetic analysis of drugs is useful for evaluation of efficacy of novel ophthalmic formulations. A simple and sensitive ultra performance liquid chromatographic (UPLC) method with ultraviolet (UV) detection was developed and validated for the quantification of dorzolamide (DZA) in rabbit aqueous humor. The aqueous humor samples were deproteinized with acetonitrile prior to UPLC analysis. A Read More

This study reports a simple and easy colorimetric method for measuring glucose consumption in tissues. Some technical parameters obtained from previous single factor tests and orthogonal designs were validated by single factor test in this study, and the kinetics of glucose consumption reaction was analyzed. Our results show that the kinetics of glucose consumption fits Michaelis-Menten equation with μmax and Km at 0 Read More

Epipodophyllotoxin and its derivatives are important derivatives of podophyllotoxin which are used in cancer chemotherapy. The conditions for the separation and identification of a mixture of seven diastereoisomer pairs at the C-2 position of epipodophyllotoxin and its esters were optimized using both high-performance liquid chromatography-diode array detection (HPLC-DAD) and high-performance liquid chromatography-el Read More