Current Pharmaceutical Analysis - Volume 10, Issue 3, 2014

Tyrosine kinase inhibitors (TKIs) are a class of targeted drugs with antiangiogenic and antitumor activities. Due to inter-individual metabolic variability, an accurate therapeutic drug monitoring (TDM) represents a key element for the patient treatment. Here a fast and easily accessible method for the quantification of 3 TKIs (with one active metabolite) in human plasma after extraction is described. Sample pre-treatme Read More

We developed and validated a rapid hydrophilic interaction chromatography (HILIC) method using a core-shell column in a conventional HPLC to assess the dissolution profile of rivastigmine hydrogen tartrate (RHT, 6 mg) from hydrophilic matrix controlled-release tablets. The dissolution profile was determined from 10-360 min in a USP dissolution apparatus II using water at 37 °C at 50 rpm. The chromatographic separati Read More

Protein phosphorylation is one of the most important post-translational modifications involved in the regulation of many cellular processes and highly efficient analytical tools are required to enrich, purify and characterize phosphoproteins. However, there are only a few analytical approaches involving phosphoprotein enrichment. The presented study describes the introduction of trivalent cerium, holmium and thuli Read More

A new simple and highly sensitive HPLC-UV method for the analysis of paclitaxel in Taxol® and Abraxane® has been developed which also allows the quantification in biological samples of pharmacokimnetic studies with a very easy sample preparation. The proposed method has advantages over previously reported methods, such as an increase in sensitivity up to 20 times, good accuracy in terms of recovery that allows to w Read More

This work aims to develop and validate a UPLC stability indicating method for quantitative analysis of Caspofungin, grounded in statistical analysis able to estimate measurement uncertainty, and determine drug degradation kinetics. The validation was performed as recommended in ICH guidelines. The chromatographic method was conducted applying the following conditions: 220 nm wavelength, 10 μL injection volume, Read More

The present work proposes a simple, accurate and low-cost method employing simultaneous detection (conductometric and potentiometric) during a precipitation titration using silver nitrate as titrant for diltiazem hydrochloride (DTZ) determination. Both methods were based on the chemical reaction between chloride ions coming from diltiazem hydrochloride molecule and Ag(I) ions, yielding the precipitate AgCl(s). The condu Read More

In this work, the development of flow system that is simple, precise and accurate for determining fluoride in mouthwash is described. The determination of fluoride was based on the on-line formation of a Fe(III)–salicylate complex, which is consumed in the presence of fluoride, generating the colourless hexafluoroferrate(III) complex ion, [FeF6]3-. The proposed procedure exhibits a linear concentration range of Read More

Nitazoxanide, 2- [(5-nitro-1, 3-thiazol-2-yl) carbamoyl] phenyl] ethanoate (NTZ) is a new broad-spectrum, antiparasitic drug. The acid, alkaline and hydrogen peroxide oxidative degradation were studied in order to analyze nitazoxanide in the presence of its degradation products using high performance liquid chromatographic assay. The liquid chromatographic separation was achieved using two columns namely the solid co Read More

Proteomic approaches posses a huge potential in understanding diseases such as cancer. These analyses rely on the extraction of proteins from clinical specimens and their subsequent enzymatic digestion in peptides prior to mass spectrometric analysis. The characterisation of chosen subproteomes – like phosphoproteome – which are rich reservoirs of potential biomarkers for disease, requires much more sophisticate Read More