Current Pharmaceutical Analysis - Volume 10, Issue 4, 2014

Caffeine is the oldest consumed psychoactive drug, present in coffee, cocoa, tea, among other products. Several conventional methods are used to analyze caffeine, for example, chromatography and spectrometry, but these methods are expensive when compared to electroanalytical methods. The objective of this study is to study the feasible applicability of electrochemical techniques for quantification of caffeine in medications Read More

We developed a method that uses flow through voltammetric sensor to quantitatively determine thiazolidine-2, 4-diones (TZDs) derivatives which are pioglitazone, rosiglitazone and troglitazone. The method uses a gold electrode. The dynamic range for determining thiazolidine-2, 4-diones (TZDs) is extended by more than two orders of magnitude. The quantification limits are 0.10, 23, 15, 0.005 ng for thiazolidine-2, 4-dione, Read More

We have developed an efficient method for fast determination and pharmacokinetic study of paclitaxel in rat plasma. The method involved a flow-injection chemiluminescence (FI-CL) technique that depends on the inhibition of a luminol/ sulfobutylether-β-cyclodextrin (SBE-β-CD) – chemiluminescence (CL) reaction system by paclitaxel. A linear relationship was found between the loss of CL and the quantities of pacli Read More

A set of 36 compounds with local anesthetics properties and with the literature values of their biological activity was used to search for relationships between structural parameters obtained from semiempirical RM1 method. Ab initio calculations have been done for molecules in vacuo and also for their hydrated form by placing the molecule under consideration in an environment of water molecules. Finally the data examined th Read More

Florfenicol is a synthetic and broad-spectrum antibiotic and developed for veterinary use. In this study, onestep membrane-based competitive colloidal gold-based immunochromatographic formats for the rapid detection of florfenicol were developed. The quality of colloidal gold particles was monitored by UV-vis spectroscopy. For the conjugation of colloidal gold and antibody, 24 μg/mL of antibody was confirmed to the op Read More

The present paper reports the development of novel, stable & validated RP HPLC method for the simultaneous estimation of Artemether (ART) and lumefantrine (LUM) in bulk and tablet dosage form. Good chromatographic separation was achieved by isocratic mode with a mixture of phosphate buffer: methanol in the ratio of 50: 50 as the mobile phase with waters symmetry Shield Rp18Column (250 mm x 4.6mm x 5) μm as stati Read More

Ticagrelor is an orally active, reversibly bind of the P2Y12 receptor that can prevent ADP-mediated platelet activation and has a faster onset of action than clopidogrel. A stability-indicating HPLC method for the determination of ticagrelor in coated tablets was developed and validated. Chromatographic analysis was performed in a Shimadzu liquid chromatograph, equipped with Phemomenex® C18 column (250 x 4.6 m Read More

Herein, we report cellulose sulfuric acid (CSA) as a heterogeneous, eco-friendly, reusable catalyst for the synthesis of β -indolylketones via conjugate Micheal addition reactions of indoles, to chalcones in acetonitrile at 80 °C for 1.5–4 h. The structures of the synthesized compounds were evaluated by spectral data. All the synthesized compounds were screened for their antimicrobial activity against bacterial (S. aureus, K. Read More

This review focuses on analytical methods for the determination of reactive oxygen species (ROS): a superoxide/ hydroperoxyl radical, hydrogen peroxide, singlet oxygen and a hydroxyl radical. The methods which are described in this paper are based on: electron paramagnetic resonance spectroscopy (EPR), nuclear magnetic resonance (NMR), mass spectroscopy (MS), spectroscopy UV-Vis, gas chromatography (GC), che Read More