Current Pharmaceutical Analysis - Volume 10, Issue 1, 2014

The studies of new anti-cancer drugs are constantly in the centre of scientific interest. This paper provides an overview of the complex compounds of platinum(II) and palladium(II) as anti-cancer drugs. These drugs have been described in dependence on the type of cells which are under the influence of the cytotoxic drug and side effects arising from the use of the drug. In the case of some drugs such as cisplatin or trans-[Pt Read More

To treat hypertension around 75% of patients will require combination therapy for which administration of lisinopril and indapamide could be of practical use. Lisinopril has a low oral bioavailability (around 25%) with large intersubject variability (6-60%), due to slow absorption. In order to improve drug permeability, encapsulation of the drugs within nanoparticles (NPs) has demonstrated great potential. For this reason we h Read More

The influence of addition of dsDNA on the electrocatalytic effect of carbon matrices, e.g. synthetic monocrystalline diamond, fullerenes C60 and multi-walled carbon nanotubes was studied. Three neurotransmitters: dopamine, epinephrine and norepinephrine, which are known as biomarkers that play an important role in transporting and modulating nervous impulses in the synapse were chosen as model analytes. Cyclic volta Read More

Transporters belonging in the ATP-binding cassette (ABC) family are crucially involved in the determination of the pharmacokinetic behavior of xenobiotics and in the development of drug resistance. The targeting of these transporters has been accepted as a rational option via which to modify the absorption or distribution of pharmaceutical agents and to combat ABC-related inefficiency. Inhibitors of the breast cancer re Read More

Capillary electrophoresis (CE) was used to follow Diclofenac tablet dissolution, in very short times and allowing dissolution testing without volume replacement. By using Student´s t test and F-test, this CE method was compared with HPLC. Statistical data show that there are no significant differences among them. The drug release kinetic of diclofenac tablets was described by various mathematical models and equations. Read More

An improved RP- HPLC method with PDA detection has been developed and validated for the simultaneous estimation of artemisinin and dexamethasone in ocular nanodispersion formulations and it has been successfully adopted to check the ex-vivo corneal transport. The chromatographic separation was achieved using phenomenex C18 column (250 × 4.6 mm, 5 μm) analytical column and the mobile pha Read More

With the objective of decreasing analysis time and retaining good efficiency (UPLC-MS/MS) is an outstanding analytical approach for speedy biomedical analysis. The aim of this study was to develop validate a simple, rapid, sensitive and specific UPLC-MS/MS method for quantification of cinacalcet, a calcimimetic agent, which acts on a calcium sensing receptor of the parathyroid gland in human plasma. After following a li Read More

Robustness tests were performed on a gradient RP-HPLC method that had been developed for rapid simultaneous separation and determination of clarithromycin, norfloxacin, doxycycline, tinidazole and omeprazole in their pharmaceutical dosage forms. Separation was carried out on a 250 x 4.6 mm (i.d.), 5μm ODS column (Inertsil, Tokyo, Japan) and multiwavelength overlay chromatograms at max of 210 and 310 nm were us Read More

DNA methylation is the most common epigenetic tool used by cells to express or silence a gene. Methylation is observed when there is an addition of methyl group at position 5 of cytosine pyrimidine ring or at nitrogen present on 6th position of adenine purine ring. It plays a vital role in many aspects of biology including development and disease. A revolution has been witnessed in DNA methylation analysis technology over the Read More