Current Pharmaceutical Analysis - Volume 10, Issue 2, 2014

A protective effect of α -ketobutyrate on hippocampal neuronal cells treated with H2O2 was tested. α - Ketobutyrate concentrations were as follows: 0.5 mM, 1 mM and 5 mM. Tests were performed at the temperature of 37 ºC. Moreover, the protective effect of α-ketobutyrate on the cytotoxicity of 100 and 40 nmol/4·103 cells treated by hydrogen peroxide was observed. Furthermore, the role of α-ketobutyrate as an e Read More

Introduction: Gastrografin® is a commercially available contrast agent, primarily utilised in abdominal computed tomography. In order to conduct a randomised controlled clinical trial to determine whether gastrografin® may confer a therapeutic benefit in a postoperative ileus it was necessary to ensure that re-packaging of gastrografin® during blinding had no effect on the stability of its active diatrizoate salts (DTZ). Aim: To de Read More

A simple, sensitive, specific, robust, precise and accurate reverse-phase high performance liquid chromatographic (RP-HPLC) method was developed and validated for suitable quantitation of fenofibrate in bulk and pharmaceutical dosage forms. The investigation of various validation parameters such as system suitability, linearity, detection limit, quantification limit, precision, accuracy, specificity, robustness and stabil Read More

Amikacin (AMK) is a semisynthetic aminoglycoside antibiotic derived from kanamycin A that lacks a strong ultraviolet absorbing chromophore or fluorophore. Greatly enhanced resonance Rayleigh scattering (RRS) signals of AMK can be observed with a conventional fluorescence spectrophotometer when AMK interacts with pontamine sky blue (PSB) and forms an ion-association complex in weakly acidic buffer me Read More

A simple, rapid and cost-effective NP-TLC method combined with densitometry was developed for determination of estradiol hemihydrate in tablet dosage form. TLC-densitometric analysis was conducted on silica gel 60F254 plates. Benzene + methanol in volume composition 9:1 was used as a mobile phase. The detection was carried out at 200 nm. Applied chromatographic conditions enabled complete separation of estradiol h Read More

A comparative investigation has been developed for the stability constants of several amino acid complexes with divalent metal ions, which have been determined by potentiometric pH titration. Depending on the metal ion–binding properties, vital differences in the building complexes were observed. The present study indicates that in some M(L) complexes, metal ions are arranged in carboxyl groups, but in other M(L)compl Read More

Stability indicating a reverse phase high- performance liquid chromatography (RP-HPLC) method for the analysis of Ketoprofen (KP) was developed and validated as per the International Conference of Harmonization (ICH) guidelines, Q1A (R2). Chromatographic separation was achieved on a Capacel Pak (Shiseido, Tokyo, Japan) C18 Type MG column (250 mm × 4.6 mm) 5 μm particle size, using isocratic elution of mobile phase Read More

In the pharmaceutical world, impurity profiling of an APIs or drug substance and products is an important aspect. It has become an important phase as per various regulatory authorities, to be carried out for all the APIs, may be because of direct relation with the efficacy and safety of drugs. For the characterization of impurities, a number of analytical techniques such as chromatographic, spectroscopic and sophisticat Read More