Current Proteomics - Volume 10, Issue 4, 2013

The detection of multiple proteins is essential not only to unraveling the mysteries of breast cancer, but also to the identification of important biomarkers and therapeutic targets relevant to breast cancer. Antibody arrays are a powerful platform for accelerating discovery in breast cancer research, allowing the researcher to rapidly detect expression profiles of multiple proteins and phosphorylation signatures. To date, anti Read More

To gain new insights into the mechanism of soybean (Glycine max) interaction with the soybean cyst nematode (Heterodera glycines), we compared protein abundance in soybean roots infected by the soybean cyst nematode at different time intervals. Proteins were extracted from roots of 3 and 8 days post-inoculation (dpi) by soybean cyst nematode Heterodera glycines and separated by 2-DE. A difference in abun Read More

Bottom-up, or shotgun, proteomics typically relies heavily on trypsin for its ease-of-use and reproducibility. Nonetheless, the need for greater sequencing depth and coverage has led researchers to develop complementary digestion approaches using other proteases. One such protease, endo-Lys-N [EC 3.4.24.20] (Lys-N), has been explored in considerable detail for its use in proteomics. Some of the advantages of Lys-N in Read More

The success of a proteomic experiment largely depends on the quality and quantity of the protein extract. Currently, various protocols are available for extraction of proteins from different types of samples; however, further optimization is required for every new sample type. Hence, a common protein extraction protocol is desirable. In the present study, soluble proteins were extracted from six diverse samples using TRIzol with Read More

Atherosclerosis is considered a chronic inflammatory disease and injury to vascular endothelial cells is an important event at the onset of its pathogenesis. Oxidative stress is regarded as a pivotal pathogenic factor in the development of endothelial cell injury and apoptosis. Tanshinone IIA is considered the main active lipophilic component of the traditional Chinese medicine Danshen. It has been demonstrated that Tanshino Read More

Countercurrent chromatography (CCC) is a unique liquid-liquid partition chromatography without any solid supports. In this paper, the progress for preparative separation of proteins and peptides by CCC has been reviewed. It covers the recent development of CCC instruments, including hydrostatic and hydrodynamic instruments, the optimization of two-phase solvent system, as well as the recent applications in separatio Read More

Cancer is one of the most important health problems in the world and the treatment approaches are still challenging and generally not at desirable levels. Conventional anti-cancer agents may cause systemic toxicity, have poor solubility, and lack of selectivity. In order to overcome these hurdles, nanoparticle based therapeutics have been widely employed to enhance therapeutic efficacies and reduce systemic toxicity of Read More