Current Proteomics - Volume 1, Issue 4, 2004

Separation of proteins from biological samples by two-dimensional polyacrylamide gel electrophoresis (2D -PAGE) followed by their identification by mass spectrometry is a widely used technique in proteomics. Two dimensional difference gel electrophoresis (2D-DIGE) is a relatively new technique in 2D-PAGE for multiplex quantitative analysis of the component proteins of related but different protein samples. In this technique, prior Read More

The proteome is defined as the set of proteins that an organism can produce. By extrapolation, the “Proteome Phenotype” corresponds to the set of proteins whose synthesis is modified (under- or over-expressed) in a genetically altered cell. This approach has been successfully used to identify co-regulated genes in some Gram positive and Gram negative bacteria but can also be carried out with cells mutated in non regulatory Read More

Microarray technology plays an increasing role in proteomic research. We give an overview about recent developments in this technology focusing on molecular interaction studies using protein and antibody microarrays. We report about technical aspects in the development of protein microarrays and describe different surfaces and detection modes. Furthermore, we review the applications of protein microarrays in diff Read More

In recent years, there has been a rapid and steady increase in the number of nucleic acid binding proteins (NABPs) and the list is continually increasing. However, a significant percentage of NABPs, the so-called hypothetical or predicted proteins has only been presumed based upon nucleic acid sequences and therefore remain to be shown to exist at the protein level. In general, neither the expression pattern analysi Read More

In the post-genome era, real-time monitoring of proteins is of great importance. The application of molecular beacons (MBs) for real time protein detection demonstrates great advantages in the understanding of many important biological processes involving two key biomolecules: nucleic acid and protein. In general, MBs are single-stranded oligonucleotide probes that have been designed to report the presence of complement Read More

This review focuses on rapid, efficient, and sensitive characterization of the proteome by capillary electrophoresis (CE) and microchip capillary electrophoresis (MCE) in conjunction with laser-induced fluorescence (LIF). A number of advanced CE-LIF and MCE-LIF techniques, including on-column concentration techniques, on-line chemical reaction and enzyme-assay techniques, integrated microdevices, and high-efficiency Read More