Current Proteomics - Volume 1, Issue 2, 2004

The inhibition of protein phosphorylation is proving to be a powerful therapeutic approach to treat a number of disorders. As more protein kinase inhibitors are developed, critical issues of drug and target specificity must be addressed. While a remarkable amount of information exists for the regulation of protein kinase function, relatively little is known about the substrates they phosphorylate. Classical protein kinase substrate Read More

Proteomics, the parallel separation, identification and quantification of all proteins produced by a tissue or cell culture, together with the analysis of protein properties like posttranslational modifications and interactions, provides more detailed information about the biological system under study than the determination of the static genome or expression profiling based on mRNA. To benefit fully from the power of proteomics, som Read More

Histological assessment of nuclear morphology is a standard procedure in clinical practice. The implications of altered nuclear structure accompanying neoplastic disease are still not fully understood. Nuclear matrix proteins were considered as structural units defining the shape of nuclei. Consequently, it was assumed that nuclei displaying characteristics of cancer cells would exhibit specific nuclear matrix proteins. Read More

Proteomic approaches play an important role in the study of complex biological systems. The application of proteomic technologies in plant science has been strongly supported by the completion of genome sequence projects of the model plants Arabidopsis thaliana and rice. This review focuses on the state of proteomic technologies with special emphasis on their application in plant biology. An overview of recent developments i Read More

The botulinum neurotoxins [BoNT] can be subdivided into immunologically distinct serotypes A-G and are multidomain proteins that are released by pathogenic strains of Clostridium botulinum. Each neurotoxin contains a catalytic domain that is a metalloprotease and cleaves a specific site on one of three proteins involved in synaptic vesicle fusion. Substrate cleavage leads to loss of neurotransmitter release and thus to paraly Read More

Fibroblasts represent one of the most widely used cell types to investigate the biology of connective tissues in normal and pathologic conditions. Aim of the present review is to emphasize, in the light of the current literature, the importance of fibroblast proteomics as a powerful resource for functional genomics in health and disease. Only very recently, proteomic techniques has been applied to characterise human dermal fib Read More