Current Proteomics - Volume 1, Issue 3, 2004

The human genome project has opened novel scientific avenues such as structural proteomics. The major challenge in structural proteomics is to predict protein structure-function relationships, including the identification of those proteins whose structures are partially or fully unknown. The use of two-dimensional gel electrophoresis and mass spectrometry methods to identify proteins strongly aids our understanding of biolo Read More

Protein arrays with ability to simultaneously detect multiple protein expression levels, protein-protein interactions, protein-DNA or RNA interactions, protein-small molecule interactions and protein modifications will be an excellent tool in biomedical discovery. A variety of technologies have been developed to meet this need. In general, these assay systems can be divided into two major categories: label-based approaches, and sa Read More

The formation of biofilms is a universal bacterial survival strategy. Biofilms occur on inert and living supports in natural environments and in industrial installations. Given their importance in various industrially relevant areas and in human health, numerous investigations have focussed on the particular physiology of these fixed microorganisms. It is now well recognised that bacteria present in biofilms behave quite different Read More

With the advent of proteomic methods, a sudden switch from genomics to proteomics has been observed and indeed, in many cases no definite conclusions can be drawn from the nucleic acid data. Moreover, there is a long and unpredictable way from RNA to protein and differences between neurodegenerative disorders at the mRNA level could not be verified at the protein level. However, proteins carry out the ultim Read More

Fertilization is the biological bridge between gametogenesis and embryogenesis, by which sperm and eggs grow and mature, mate, and fuse with each other to give rise to the birth of a new individual(s). In this review, we address the current status of the knowledge in the field of fertilization, with a special focus on the role of maternal and paternal protein molecules such as tyrosine kinases, phospholipase C, and inositol trisph Read More

This review covers the vast array of methods that appeared in the last few years for separation and identification of thylakoid membrane proteins present in chloroplast, a good model for setting up analytical methods suitable for membrane proteins. Although the major method for protein separation is gel electrophoresis (GE), we will summarise in this review the results of several studies performed to develop a rapid and s Read More