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Volume 21, Issue 1
  • ISSN: 1573-4072
  • E-ISSN: 1875-6646

Abstract

Background

The study addresses the underexplored realm of cytotoxicity evaluation involving binary mixtures of leaf extracts and Titanium nanoparticles (TiONPs). The focus lies on understanding the combined effects of these components on cancer cells (A549, U937, and HeLa).

Methods

cytotoxicity assessments were employed to evaluate the toxicity of leaf extracts, TiONPs, and their combination. The study utilized MTT, NRU, and LDH assays to measure cellular viability. Additionally, reactive oxygen species (ROS) and glutathione levels were assessed alongside the aforementioned assays.

Results

The toxicity percentage exhibited dose-dependent behavior for leaf extracts, TiONPs, and their combination. Interestingly, when leaf extract and TiONPs were combined, the reduction in cell viability was noticeably more than when the exposures were made separately. Moreover, the production of ROS was higher in the combined toxicity scenario, and a more pronounced decrease in glutathione levels was observed compared to individual exposures.

Conclusion

The findings suggest that the combined effects of leaf extract and TiONPs induce greater cytotoxicity compared to their impacts. This underscores the potential for synergistic cytotoxicity in combined exposure scenarios, warranting further exploration of combined effects in future studies.

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2024-03-19
2025-01-17

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/content/journals/cbc/10.2174/0115734072294581240311064402
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Individual and Dual Cytotoxicity of the Combination of Passiflora Caerulea Leaf Extract and Titanium Oxide Nanoparticles against A549, U937, and Hela Cells
Curr. Bioact. Compd. 21, E190324228136 (2025); https://doi.org/10.2174/0115734072294581240311064402
/content/journals/cbc/10.2174/0115734072294581240311064402
/content/journals/cbc/10.2174/0115734072294581240311064402
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  • Article Type:     Research Article
Keyword(s): A549; HeLa cells; Passiflora caerulea; reactive oxygen species; titanium nanoparticles; U937

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