Current Analytical Chemistry - Volume 14, Issue 5, 2018

Background: Metformin (Mf) (N,N'-Dimethylbiguanide) is used as the first-line medication for treating diabetes mellitus type II. It is also considered as a “gold standard” treatment for various diseases like hyperglycemia, insulin resistance, infertility, cardiovascular disease, renal dysfunction, polycystic ovary syndrome, obesity, cancer and ageing. Majority of the methods available for quantitative analysis of Mf are expensive, t Read More

Background: A cationic polyelectrolyte, polyhexamethylene biguanide hydrochloride (PHMB), is widely used for disinfectants in personal-care products for cosmetics and toiletries. Several colloidal titration methods for the determination of PHMB are very tedious and time-consuming. A Flow Injection Analysis (FIA) is a very advantageous analytical method because of its ease of handling, high reproducibility and high sam Read More

Background: The development of nanomaterials modified electrode can provide more opportunities for the enhancement of the analytical performance in constructing electrochemical sensor and biosensor. LiFePO4 (LFP) is a promising candidate to develop new modified electrodes owing to its advantages such as cheap cost, environmental compatibility, high safety, non-toxicity, large theoretical capacity, long cycle l Read More

Background: Microscale in vitro assays are fast, simple, and inexpensive, with reduced reagent quantities, waste, and experimental animal use. However, they have low reproducibility and low correlation with the results of in vivo models, possibly due to differences in precision and accuracy in methodologies between laboratories. Objective: The objective was the optimization and validation of an in vitro assay, carried out on Read More

Background: Honeysuckle, as one of the most important traditional Chinese medicines (TCMs), has attracted more and more attention due to the increasing number of people using it as selfmedication or alternative therapy. In order to determine whether honeysuckle is a special matrix that may be less susceptible to be infected by toxigenic molds and consequently to less mycotoxins contamination due to its biological and p Read More

Background: Molecularly Imprinted Polymers (MIPs) are used as artificial receptors in biosensors for the detection of a wide range of analytes from small drug molecules to large molecular weight biomolecules. Quantification of Digoxin (DIG) in human serum and pharmaceutical samples has very high importance due to its low margin of safety. In this report, a highly sensitive molecularly imprinted polymer for DIG is prepared Read More

Background: Considering the economic loss by columnaris disease in fish, a rapid, simpleto- use and visual detection technology for the detection of Flavobacterium columnare (F. columnare) is very important. Methods: This assay utilizes LAMP for F. columnare-specific target gene amplification, and the resulting LAMP amplicons were further analyzed by Immunochromatographic Strip (ICS) for the rapid visual detection of F. colu Read More

Background: Multivariate transfer techniques have become a widely accepted concept over the past few years, since they avoid full recalibration procedures when instruments are changed to analyze a specific sample. Objective: This paper reports a multivariate control chart transfer approach between two near infrared (NIR) spectrometers for simultaneous determination of rifampicin and isoniazid in pharmaceutical f Read More

Background: Imatinib (Gleevec) is an antineoplastic agent acting as tyrosine kinase inhibitor used for the treatment of cancer such as lymphoblastic leukemia. The dosage of the anticancer drugs plays a critical role in the survival of the patients. Therefore, each patient should be administered with a customized dosage of the antineoplastic agent. For this reason, patients' plasma and urine samples should be monitored to obtain Read More

Background: Two enantiomers of a drug which might have various pharmacological, physiological, and toxicological properties are well-known. Enantioseparation of chiral drugs is becoming significant in various fields. Objective: The aim was to obtain better detection sensitivity and good enantioresolution of Norepinephrine (NE), Epinephrine (EP) and Isoproterenol (ISO)at the same time. Method: A new and sensitive meth Read More

Background: Glutathione peroxidase (GSH-Px) is one of the major enzyme in the antioxidative defense mechanism present in the cells. Methods: In the present study a novel and simple microplate-based method was developed, using the cupric neocuproine complex (Cu(Nc)2 2+) as a chromogenic oxidizing reagent, for the assessment of GSH-Px activity of biological samples for the first time. In this GSH-Px enzyme acti Read More

Background: The ICH guidelines Q8 (Analytical Quality by Design (AQbD) technique) give the procedure for optimization of analytical parameter involved method development and validation of Sirolimus (SL) in spiked Plasma and in dosage form by using advanced analytical technique. Objective: This research paper is based on Quality by Design (QbD) finalized conditions for a method for the determination of concentrat Read More

Background: This paper is focused on application of a recently developed disposable array of carbon composite electrodes and carbon paste electrode for the determination of indoxyl sulphate. Results: Measurement of basic electrochemical behaviour of indoxyl sulphate on working electrode resulted in the selection of BR buffer pH 2.0 and pH 3.0 as the optimum medium for carbon composite film electrode and carb Read More