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Volume 1, Issue 1
  • ISSN: 2666-6499
  • E-ISSN: 2666-6502

Abstract

Background

Probiotics are live microorganisms that, when administered, have beneficial effects on human health. Hence, various probiotic organisms belonging to the genera and are being increasingly used in food and pharmaceutical products. For regulatory compliance, simple and reliable analytical methods are required for selective enumeration of different probiotic organisms present in food and pharmaceutical formulations.

Objective

This study aimed to establish a rapid PCR-based method for the identification and differentiation of and strains.

Methods

Species-specific and genus-specific primers for and were designed based on their 16S rRNA gene sequence. Genomic DNA of different and strains were subjected to PCR with annealing temperature specific for respective primer pairs.

Results

primers gave positive PCR results only with strains and not with other species, whereas genus-specific primers gave positive PCR results with most of the strains.

Conclusion

The three species-specific primer pairs and three genus-specific primer pairs of used in this study could easily identify and differentiate and with high specificity.

© 2024 Bentham Science Publishers
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2024-08-29
2024-11-23

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/content/journals/probiot/10.2174/0126666499306488240823105015
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Rapid Identification of Lactobacillus acidophilus and Bifidobacterium by a 16S rRNA PCR Method
Curr. Probiotics 1, E26666499306488 (2024); https://doi.org/10.2174/0126666499306488240823105015
/content/journals/probiot/10.2174/0126666499306488240823105015
/content/journals/probiot/10.2174/0126666499306488240823105015
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  • Article Type:     Research Article
Keyword(s): 16S rRNA; Bifidobacterium; Lactobacillus; PCR; pharmaceutical formulations; Probiotics

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