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- Volume 23, Issue 9, 2016
Protein and Peptide Letters - Volume 23, Issue 9, 2016
Volume 23, Issue 9, 2016
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Synthesis and Characterisation of Substrate-Based Peptides as Inhibitors of Histone Demethylase KDM4C
The design and synthesis of modified pentapeptides based on a truncated version of the substrate for KDM4C, a histone lysine demethylase (KDM), and investigation of their inhibitory activity at KDM4C is reported. By modifying the lysine residue corresponding to lysine 9 at histone 3 (H3K9), three different series of peptides were designed and synthesized. One series contained N-acylated H3K9 and two series introduced triaz Read More
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Expression, Purification and Characterization of Recombinant Canine FGF21 in Escherichia coli
Authors: Zhong Zhenga, Chengjun Yanga, Ruofeng Yinb, Jinxi Jiangc, Haiting Hea, Xinxin Wanga, Mujie Kan and Yechen XiaoThe canine metabolic diseases, such as obesity and diabetes, have become a worldwide problem. Fibroblast growth factor 21 (FGF21) is a potent regulator which has many biological functions relative to metabolism regulation. It suggests that FGF21 plays important roles in regulating canine metabolic diseases. To acquire the recombinant canine FGF21 (rcFGF21) in Escherichia coli, the recombinant bacteria were induced by 0 Read More
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Molecular Interactions of an Ornithine-Rich pH-Responsive Self- Assembling Peptide with a Model Lipid Membrane: Conformational Aspects
Authors: Glauber G. Nascimento, Luiz C. Salay, Paulo R.S. Sanches and Eduardo M. CilliInvestigating interactions of designed peptide-based biomaterials with lipid membranes is important for applications in nanobiotechnology. Here the interaction of an ornithine-rich pH-responsive peptide called P11-5 with a model membrane was investigated employing Fourier transform infrared spectroscopy (FTIR). The results showed that in the range of pH 6.0-8.0 the peptide P11-5 shows spectral features which are evidence of Read More
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Alanine Counteracts the Destabilizing Effect that Urea has on RNase-A
Authors: Rimpy K. Chowhan, Fasil Ali, Mohd. Y. Bhat, Safikur Rahman, Laishram R. Singh, Faizan Ahmad and Tanveer A. DarBackground: It is generally believed that organisms use and accumulate methylamine osmolytes to prevent urea’s damaging effect on protein stability and activity. However, urea-rich cells not only accumulate methylamines but also many other methylated and non-methylated compounds as well. But, so far it is not known whether osmolytes that are not accumulated in urea-rich cells could also confer urea-counteracting pr Read More
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Spectroscopy and Molecular Modeling Study on Binding of Nickel Phthalocyanine to Human Serum Albumin
Authors: Hamid Dezhampanah, Roghaye Firouzi and Leila HasaniThe interaction of nickel tetra sulfunated phthalocyanine( NiTSPc) with human serum albumin (HSA), in 20 mM phosphate buffer pH 7.4 was investigated using advanced techniques including fluorescence, synchronous fluorescence, Fourier transform infrared (FT-IR), circular dichroism (CD) spectroscopy and molecular docking. The fluorescence quenching measurements showed a single binding site on HSA for NiTSPc w Read More
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Identification of SER-PRO-CYS Peptide in Blood Serum of Multiple Sclerosis Patients
Monitoring of multiple sclerosis (MS) requires additional molecular markers. Recently, we used original TCA-precipitation/extraction approach in combination with MALDI TOF/TOF mass-spectrometry and identified earlier unknown 48 kDa form of the unconventional myosin IC isoform b (Myo1C) in blood serum of the MS patients. Further examination of TCA-extracted fraction of blood serum of these patients by means of Read More
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Polyacrylamide Gel-Entrapped Fungal Manganese Peroxidase from Ganoderma lucidum IBL-05 with Enhanced Catalytic, Stability, and Reusability Characteristics
Authors: Muhammad Bilal, Muhammad Asgher and Hafiz M. N. IqbalIn the present study, polyacrylamide gel (PAG) was utilized as bolster material for the immobilization of in-house extracted and partially purified manganese peroxidase (MnP) through an entrapment technique yielding significant MnP immobilization (87.3±3.3 %) and remarkable stability of the enzyme (37.2±2.4 %) after a storage period of two months at 4°C. The immobilization also increased the optimal temperature by 10 Read More
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Cell-Free Technologies for Proteomics and Protein Engineering
Authors: Hiroyuki Ohashi and Etsuko Miyamoto-SatoCell-free translation systems facilitate rapid production of specific proteins and are particularly suited as high-throughput methods for whole-genome protein synthesis. Moreover, these systems do not rely on living cells, thereby allowing the synthesis of unstable or cytotoxic proteins in vitro. In this review, we describe the principles and potential applications of cell-free protein translation systems and the future prospect Read More
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Cellular Functions of RNA-Binding Motif Protein 3 (RBM3): Clues in Hypothermia, Cancer Biology and Apoptosis
Authors: Heyam I. M. Al-Astal, Maram Massad, Manaf AlMatar and Harith EkalCell rapidly adapts to the harsh environment under certain conditions such as hypothermia and stress via activity of certain specific cold-shock proteins. Two cold shock proteins, RNA-binding motif protein 3 (RBM3) and coldinducible RNA binding proteins (CIRP), have been unveiled in human cells. RBM3, which is able to regulate their expression at the level of translation by binding to different transcripts, allows the cell to resp Read More
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Molecular Remodeling of the Insulin Receptor Pathway by Thiazolidinediones in Type 2 Diabetes Mellitus: A Brief Review
Authors: Nikhil S. Sahajpal and Subheet K. JainType 2 diabetes mellitus (T2DM) is characterized by abnormalities in carbohydrate, lipoprotein and lipid metabolism, leading to hyperglycemia and several other complications. Insulin is the major hormone regulating these facets by eliciting various biological responses through its receptor. Insulin exerts diverse effects on cells by targeting distinct functions such as gene expression, fatty acid synthesis, glucose transport and Read More
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Scorpion Toxin Polyptides as Therapeutic Agents: An Overview
Authors: Janardhan Bhavya, Niyonzima N. Francois, Veena S. More and Sunil S. MoreScorpions are distributed throughout the world and numerous biological molecules are found in their venom most importantly peptide toxins. These toxins modulate the ion channels either by blocking the pore of the channel or by altering the voltage gating. Molecules which block the pores have been useful in deciphering the structure of the ion channels. Many scorpion toxins have already been used for probing the voltage gated Read More
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Volumes & issues
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Volume 32 (2025)
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Volume 31 (2024)
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Volume 30 (2023)
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Volume 29 (2022)
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Volume 28 (2021)
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Volume 27 (2020)
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Volume 26 (2019)
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Volume 25 (2018)
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Volume 24 (2017)
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Volume 23 (2016)
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Volume 22 (2015)
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Volume 21 (2014)
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Volume 20 (2013)
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Volume 19 (2012)
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Volume 18 (2011)
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Volume 17 (2010)
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Volume 16 (2009)
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Volume 15 (2008)
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Volume 14 (2007)
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Volume 13 (2006)
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Volume 12 (2005)
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Volume 11 (2004)
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Volume 10 (2003)
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Volume 9 (2002)
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Volume 8 (2001)
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