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Volume 13, Issue 2
  • ISSN: 2211-7385
  • E-ISSN: 2211-7393

Abstract

Background

This study investigated the potential of Plant-Derived Exosome-Like Nanoparticles (PDENs) as cosmeceutical nanocarriers for treating skin problems, such as scar removal, face rejuvenation, anti-aging, and anti-pigmentation.

Objectives

Researchers isolated PDENs from Yam Bean () using PEG-based precipitation, gradual filtration, and various centrifugations at low temperatures. Followed by and studies using HDF cells and Zebrafish.

Methods

The morphology of the YB-PDENs was determined using TEM analysis, they had a spherical shape with diameters of 236,83 ± 9,27 nm according to PSA. The study found that YB-PDENs were stable in aquabidest at 4°C for one month of storage and had ~-26,5 mV of Zeta Potential. The concentration of YB-PDENs was measured using the BCA Assay, and internalization of YB-PDENs to HDF cells was observed using a Confocal Laser Scanning Microscope labelled with PKH67.

Results

As for cytotoxicity, after 24 and 72 hours of incubation with YB-PDENs, the viability of HDF cells remained more than 80%. The study also examined cell migration using the Scratch Assay and found that at 2,5 μg/mL, YB-PDENs had better migration results than other concentrations. Immunocytochemistry showed that collagen expression was higher after 14 days of incubation with YB-PDENs, and melanocytes in zebrafish decreased at each concentration compared with controls.

Conclusion

In conclusion, this study is the first to extract and describe PDENs from Yam Bean (), with YB-PDENs having a promising anti-melanogenic effect in skin treatment. This study highlights the potential of YB-PDENs as a promising alternative to depigmentation and skin whitening treatments.

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2025-06-20

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/content/journals/pnt/10.2174/0122117385279809231221050226
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Characterizing Nanoparticle Isolated by Yam Bean (Pachyrhizus erosus) as a Potential Agent for Nanocosmetics: An in vitro and in vivo Approaches
Pharm. Nanotechnol. 13, 341 (2025); https://doi.org/10.2174/0122117385279809231221050226
/content/journals/pnt/10.2174/0122117385279809231221050226
/content/journals/pnt/10.2174/0122117385279809231221050226
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  • Article Type:     Research Article
Keyword(s): analysis; anti-melanogenic activity; Exosome; Pachyrhizus erosus; plant-derived exosome-like nanoparticle; YB-PDENs

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