Evaluation of Anti-Diabetic and Anti-Hyperlipidemic Activity of Isolated Bioactive Compounds of Leaves of Annona reticulata Linn.

Background: The leaves of Annona reticulata Linn. have been traditionally used by the tribes of Assam as a source of medicine to mitigate a range of health ailments, including diabetes and obesity. Objectives: The current study aimed to evaluate the anti-diabetic and anti-hyperlipidemic potential of bioactive fractions isolated from the methanolic extract of Annona reticulata Linn. leaves using Nicotinamide + Streptozotocin (60 mg/kg, i.p.) induced diabetic rats. Methods: The partially purified bioactive fractions, namely F1, F2, F3 and F4 were administered to diabetic rats with a dose of 200 mg/kg, per oral (p.o.) and the effect of the fractions on serum glucose was studied up to 21 days. The potent fractions were further subjected to spectral analysis for the identification of the isolated active compounds. Results: The in-vivo anti-diabetic activity of the isolated fractions F2 and F3 was found significant controlling blood glucose level, alike glibenclamide. Interestingly, F2 and F3 treated animals were found significant in restoring the lipid and liver enzymes profile in streptozotocin challenge rats. Further, spectral analysis revealed that F2 and F3 were comprised of Quercetin and Gallic acid, respectively. Conclusion: The outcome of finding demonstrates the anti-diabetic and anti-hyperlipidemic potential of the isolates/fractions of A. reticulata, which were found enriched in polyphenolics, including Quercetin and Gallic acid; and provided logistic behind the traditional use of the A. reticulata against diabetes and obesity.