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Volume 21, Issue 17
  • ISSN: 1570-1808
  • E-ISSN: 1875-628X

Abstract

Background and Objectives

is a foodborne pathogen that is a major concern for global health. Flagellin (FliC) is an essential protein in for both motility and virulence and is a key component of flagella. Hence, focusing on FliC protein is a promising strategy for developing new anti- agents. a medicinal plant, shows promising antimicrobial properties. Thus, this study explores the therapeutic potential of against FliC protein using computational methods in comparison to the standard antibiotic ciprofloxacin.

Methods

Molecular docking simulation was performed to evaluate the binding affinity and interaction pattern of bioactive compounds present in and ciprofloxacin against FliC protein. This study also analysed protein stability and dynamics studies of the apoprotein, ciprofloxacin, kesselringine, and gloriosine complexes using molecular dynamic (MD) simulation. The MMGBSA method computed binding free energies.

Results

Through docking simulations, it was found that gloriosine and kesselringine have strong binding affinity with FliC protein, similar to ciprofloxacin. MD simulation showed consistent protein-ligand complexes during the entire simulation. The MMGBSA analysis confirmed the positive interactions observed in the docking results, showing binding free energies similar to ciprofloxacin.

Conclusion

This study suggests that the phytocompounds of shows promising therapeutic potential as a source for creating anti- agents that target FliC protein. These results suggest that may have therapeutic potential against infections and should be further studied through and experiments.

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2025-06-20

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/content/journals/lddd/10.2174/0115701808334616240822055800
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Therapeutic Potential of Colchicum luteum against Flagellin (FliC) in Salmonella typhimurium: An In silico Approach
Letters in Drug Design & Discovery 21, 3969 (2024); https://doi.org/10.2174/0115701808334616240822055800
/content/journals/lddd/10.2174/0115701808334616240822055800
/content/journals/lddd/10.2174/0115701808334616240822055800
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  • Article Type:     Research Article
Keyword(s): Colchicum luteum; Dynamic simulation; FliC; MM/GBSA; Molecular docking; Salmonella typhimurium

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