Immunomodulatory Properties of Synthetic Imidazolone Derivatives

Thirteen imidazolone derivatives were synthesized and characterized by 1H NMR, EI, IR and UV spectroscopic and CHN analysis. Among the compounds tested, two compounds, 3-hydroxy-2-phenyl-5-[Ephenylmethylidene]- 3,5-dihydro-4H-imidazol-4-one (12) and 4-[E-(5-methyl-2-furyl) methylidene]-1,2- diphenyl-1H-imidazol-5-one (13) shown to have suppressing activities on the oxidative burst of neutrophils. These two compounds were then further investigated for their effects on different cellular immune responses including neutrophils, phagocytosis and chemotaxis activities of T-cell proliferation, and cytokine production from mononuclear cells. The results demonstrate that compounds 12 and 13 inhibit reactive oxygen species (ROS) generation and phytohemagglutinin (PHA)-induced T-cell proliferation in a dose dependent manner.