Hepatoprotective Effects and Antioxidant Potential of Polyherbal Formulation Against CCl4- Induced Hepatic Injury in Albino Rats

To investigate the hepatoprotective and in vitro antioxidant activity of aqueous methanolic extract (AME) of polyherbal formulation of Calotropis procera (C. procera), Gymnema sylvestre (G. sylvestre) and Lawsonia inermis (L. inermis) leaves. The activity of the extracts was assessed on the basis of improvement in the altered level of various biochemical parameters in the liver of the rats. The extracts were also investigated for its antioxidant potential by in vitro DPPH radical scavenging assay. The results of present studies strongly indicate that the hepatoprotective and extracts assessed for DPPH free radical scavenging activity, the maximum activity was shown by polyherbal formulation with IC50 value 62.76 μg/ml. Treatment with polyherbal formulation with decreased SGPT, SGOT, ALP, TB and Cholestrol levels in serum demonstrates the hepatoprotective effect of the formulation. Polyherbal formulation revealed excellent hepatoprotective and antioxidant profile as compared to individual plants due to synergistic effect produced by different phytochemicals present in the formulation.