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image of Exploring Biological Activities of a Thai Traditional Remedy Called “Ruean-Mhoon-Nok” and its Plant Ingredient Extracts for the Treatment of Dermatitis
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Abstract

Background

Ruean-Mhoon-Nok (RMN) remedy has been used to treat skin inflammatory diseases (, dermatitis and psoriasis). However, its bioactivities related to traditional use remain unclear.

Objective

To investigate the biological activities related to dermatitis treatment of the RMN and its plant ingredient extracts, including the determination of bioactive compounds and stability study.

Methods

anti-inflammatory activities were assessed through the inhibition of NO using Griess reagent in RAW 264.7 cells, as well as IL-6 and TNF-α production using an ELISA test kit. The anti-allergic activity was performed degranulation assay in RBL-2H3 cells. The microtiter plate-based antibacterial assay was used to assess MIC and MBC. The bioactive compound in the RMN extract was measured by HPLC, while its stability was evaluated under accelerated storage conditions.

Results

The RMN extract exhibited a potential inhibitory effect on NO and IL-6 production, while it had a limited effect on inhibition of β-hexosaminidase release. In addition, the RMN extract displayed antibacterial activity against Gram-positive bacteria, including , and MRSA. Among individual plants, extract displayed outstanding results in all assays compared to the others. The HPLC results confirmed that hydroxychavicol is a major RMN extract constituent, demonstrating potent inhibitory activity on NO and IL-6 productions. However, the RMN extract was unstable when stored under accelerated conditions.

Conclusion

The RMN remedy and its bioactive compound, hydroxychavicol, have highly promising anti-inflammatory and anti-bacterial properties that might support its traditional use. However, further investigations related to the pathogenesis of dermatitis are required, including preclinical and clinical studies.

© 2025 The Author(s). Published by Bentham Science Publishers
This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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2025-03-04
2025-03-28

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/content/journals/ctm/10.2174/0122150838337932241218172008
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Exploring Biological Activities of a Thai Traditional Remedy Called “Ruean-Mhoon-Nok” and its Plant Ingredient Extracts for the Treatment of Dermatitis
Bentham Science Publishers ; https://doi.org/10.2174/0122150838337932241218172008
/content/journals/ctm/10.2174/0122150838337932241218172008
/content/journals/ctm/10.2174/0122150838337932241218172008
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  • Article Type:     Research Article
Keywords: hydroxychavicol ; dermatitis ; anti-allergy ; Thai traditional medicine ; Ruean-Mhoon-Nok remedy ; antimicrobial ; anti-inflammation

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