Current Pharmaceutical Analysis - Volume 19, Issue 4, 2023

Multi-omics approaches have developed as a profitable technique for plant systems, a popular method in medical and biological sciences underlining the necessity to outline new integrative technology and functions to facilitate the multi-scale depiction of biological systems. Understanding a biological system through various omics layers reveals supplementary sources of variability and probably inferring the Read More

Background: After the hydrolysis of daptomycin in deuterated hydrochloric acid, the deuterium-substituted kynurenine was found, but the structure of deuterium-substituted kynurenine has not been reported. Introduction: The deuterium-substituted kynurenines were simply synthesized and confirmed to be tri- and tetra-substituted products by high resolution mass spectrum and NMR. In further, the deuterium-substituted kyn Read More

Background: Daphnoretin, as a known bicoumarin compound that contained various pharmacological activities, was isolated from Wikstroemia indica C.A. Mey (RWI). Objective: The study aims to investigate the pharmacokinetic characteristics of daphnoretin from RWI ethanol extracts in rat plasma and to determine daphnetin in rat plasma and various tissues by a rapid, reliable and sensitive ultra high performance liquid chr Read More

Objective: This study aims to investigate the main metabolites and metabolic pathways of Portulacatone B in rats, which is an alkaloid isolated from Portulaca oleracea L. Methods: Portulacatone B was administered through the tail vein of the rat, and the orbital blood at 10 and 30 min and urine and feces within 24 h were collected. The metabolites and metabolic pathways in the rat were researched by ultra-high performance liq Read More

Background: In intensive care units intravenous medicine may be used in simultaneous infusion in the same intravenous site. Sometimes, the physical compatibility and stability of the combined solutions are unknown. Objective: The objective was to develop, optimize and validate a simple, fast and sensitive stability- indicating high-performance liquid chromatography (HPLC) for simultaneous quantification of binary mixtures Read More

Aims: Flavonoids naturally exist in plants as secondary metabolites. In this study, the aim is to determine and compare the theoretical and in vivo chemical activities of 7,8- dihydroxyflavone (7,8-DHF) and 4'dimethylamino-7,8-dihydroxyflavone (4’-DMA-7,8-DHF), tyrosine receptor kinase B (TrkB) receptor agonist flavonoid molecules with reported potent neuroprotective effects. Methods: The density functional theory (DFT) ( Read More

Objective: Controlling coccidiosis disease in poultry is significant for healthy breeding, but antibiotic residues can pose a great threat to food safety, the ecological environment, and human health. A special anticoccidial drug called Hainanmycin has been widely used in Asian countries for many years, while a few studies focus on its detection. In this study, we established a hydrophiliclipophilic balance (HLB) purification and ultra- Read More

Background: To control the quality of various butyl rubber closures and assess their applicability to cefamandole nafate, HPLC/ GC-MS methods were developed to qualitatively and quantitatively analyze the organic components in various types of butyl rubber closures and drug powder of cefamandole nafate for injection, and migration of these components from rubber closures to drug also were studied. Materials and Met Read More

Background: Botulinum neurotoxins (BoNTs) are among the most potent toxins known and are also used for therapeutic and aesthetic applications. Objective: An alternative in vitro cell culture bioassay based on the induction of apoptosis on T- 47D breast cancer cells, after exposure to BoNTA, was developed and validated. Methods: The T-47D cells (ATCC HTB-133) were seeded at a density of 3 × 105 cells mL-1, and the bioassa Read More