Establishment of HLB-UPLC-MS/MS Method to Determine Hainanmycin Sodium in Chicken Liver

Objective: Controlling coccidiosis disease in poultry is significant for healthy breeding, but antibiotic residues can pose a great threat to food safety, the ecological environment, and human health. A special anticoccidial drug called Hainanmycin has been widely used in Asian countries for many years, while a few studies focus on its detection. In this study, we established a hydrophiliclipophilic balance (HLB) purification and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method to determine the hainanmycin in chicken liver. Methods: It was extracted from the sample with 0.1% formic acid-methanol (10+90, v/v) and then it was purified on a hydrophilic-lipophilic balance (HLB) cartridge. Chromatography separation was performed on an RP-C18 column, and detection of the hainanmycin was done by positive electrospray ionization (ESI.) under the multiple reaction monitoring (MRM) modes. Results: The quantification ion pair was m/z 907.5 and m/z 846.4. The calibration curve showed excellent linearity in the range of 10 to 1000 μg·L^-1, with a correction coefficient of 0.99. When spiked at 5, 100, and 500 μg·kg^-1, the recoveries ranged from 80.82% to 88.09%, with the intra-day and interday coefficients of variation from 10.76% to 13.48% and from 10.80% to 12.71%, respectively. The limit of quantification (LOQ) and detection(LOD) were 5.05 μg·mL^-1 and 1.55 μg·mL^-1, respectively. Conclusion: This method showed high sensitivity and accuracy for determining hainanmycin in chicken liver.