Current Pharmaceutical Analysis - Volume 15, Issue 2, 2019

Background: Lamivudine was approved by Food and Drug Administration of the United States for the treatment of both HIV and HBV infection, which has been widely used as monotherapy or a component of combination therapy in clinics in many countries and nationalities. Methods: In this paper, the recent chromatographic and mass spectrometry analytical methods for the determination of lamivudine individually or combi Read More

Microanalytical systems have aroused great interest because they can analyze extremely small sample volumes, improve the rate and throughput of chemical and biochemical analysis in a way that reduces costs. Microchip Electrophoresis (ME) represents an effective separation technique to perform quick analytical separations of complex samples. It offers high resolution and significant peak capacity. ME is used in many area Read More

Background: Imatinib (IM) is a chemotherapy medication metabolized by CYP3A4 to Ndesmethyl imatinib (NDI), which shows similar pharmacologic activity to the parent drug. Although methods for determination of IM and/or NDI have been developed extensively, only few observations have been addressed to simultaneously determine IM and NDI in biological tissues such as liver, kidney, heart, brain and bone marrow. Metho Read More

Background: Jingzhiguanxin (JZGX) tablet, a traditional Chinese prescription, is commonly used for treating coronary heart disease and angina pectoris in the clinic. There are six active components (Danshensu (DSS), Protocatechuic aldehyde (PD), Paeoniflorin (PF), Ferulic acid (FA), Salvianolic acid B (Sal B) and Tanshinone IIA (TA)) in JZGX tablet. Objective: In this paper, a simple and reliable method was used for sim Read More

Background: The carbamide peroxide is the most commonly active ingredient used for home dental whitening products, its quantification in pharmaceutical products is of extreme importance due to the relation with the products potency and the previously related low carbamide peroxide stability. Once, there is only one official carbamide peroxide determination based on iodometric titration, this method is time-c Read More

Background: Lipid-Core Nanocapsules (LNC) containing co-encapsulated ρ-coumaric acid and coumarin are under development. However, there is a lack of analytical methods to assay these bioactives in nanoformulations. Objective: The aim of this study was to validate an LC analytical method for the simultaneous determination of ρ-coumaric acid and coumarin in lipid-core nanocapsules. Methods: The mobile phase was compo Read More

Background: Quantification of preservatives in herbal formulation, simultaneously by high performance liquid chromatography analysis is very complex and involves series of steps including sample preparation, selection of suitable mobile phase and its validation for routine applications. Introduction: Application of Quality by Design (QbD) in the development of novel, simple, accurate and precise RP-HPLC method for con Read More

Background: A gas chromatography and mass spectrometry (GC/MS) procedure was developed and validated for the evaluation and quantification of pregabalin (PGN) in pharmaceutical preparations. Introduction: Pregabalin is a γ-amino-n-butyric acid derivative used as an antiepileptic drug for the management of fibromyalgia, and has analgesic, anxiolytic, and anticonvulsant activities. Few studies have been reported on the det Read More

Background: In this study, we have developed a novel, rapid enzymatic hydrolysis method for conversion of omega lipids (omega fatty acid triglycerides, phospholipids, omega conjugates) in to free fatty acids at room temperature using lipase and esterase enzymes. Objective: To develop simple enzymatic hydrolysis and rapid sample extraction method for quantification of free (un-esterified) and conjugated (esteri Read More

Introduction: Deltaline, an aconitine-type alkaloid, was detected in mouse blood using an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method, and the pharmacokinetics of deltaline following intravenous administration in mice was studied. Materials and Methods: The gelsenicine was used as the internal standard (IS). Deltaline and IS were eluted at a flow rate of 0.4 ml/min and separated Read More

Background: Cinacalcet is one of the second-generation calcimimetics which consists of a chiral center. The pharmacological effect of R-cinacalcet is 1000 times greater than that of the Scinacalcet. As mentioned in many literatures, 1-(1-naphthyl)ethylamine is used as the starting material for the synthesis of cinacalcet. The absolute structure of cinacalcet is influenced by the starting materials. Methods: We present the chiral s Read More