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Volume 20, Issue 7
  • ISSN: 1573-4129
  • E-ISSN: 1875-676X

Abstract

Background

D. Don, a perennial herb from the Lamiaceae family, is renowned for its medicinal properties, with scutellarin and scutellarein being key bioactive constituents.

Aim

In this study, we present the development and validation of a High-Performance Thin-Layer Chromatography (HPTLC) method for the simultaneous quantitative and qualitative analysis of scutellarin and scutellarein in the hydroethanol extract of D. Don.

Methods

The chromatographic conditions were optimized using different solvent systems, and validation was performed as per ICH guidelines.

Results

The mobile phase comprising Ethyl acetate: methanol: formic acid: water (20: 2.7: 0.5: 2) and scanning wavelength set at 254 nm was optimized for study, resulting in distinct and well-separated spots for scutellarin and scutellarein with Rf values of 0.31 and 0.96, respectively. The method was rigorously validated to ensure reliability and reproducibility. Calibration curves exhibited excellent linearity for both scutellarin (r2 > 0.98) and scutellarein (r2 > 0.99) over a concentration range of 10-30 µg/ml. The developed method underwent thorough validation following ICH guidelines. Validation parameters included accuracy, limit of quantification, limit of detection, linearity, precision, and recovery. The determined concentration of scutellarin and scutellarein in the hydroethanolic extract of D. Don was found to be 27.00 µg/mg and 20.16 µg/mg of extract, respectively.

Conclusion

This proposed method demonstrates its utility for both qualitative and quantitative analyses of scutellarin and scutellarein in the hydroethanolic extract of D. Don, offering a reliable and validated approach for quality control and standardization in the herbal product industry.

© 2024 Bentham Science Publishers
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2024-08-13
2025-01-22

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References

  1. WangL. ChenW. LiM. ZhangF. ChenK. ChenW. A review of the ethnopharmacology, phytochemistry, pharmacology, and quality control of Scutellaria barbata D.Don. J. Ethnopharmacol.202025411226010.1016/j.jep.2019.112260 31577937
     [Google Scholar]
  2. XieY. SunG. TaoY. ZhangW. YangS. ZhangL. LuY. DuG. Current advances on the therapeutic potential of scutellarin: An updated review.Nat. Prod. Bioprospect.20241412010.1007/s13659‑024‑00441‑3 38436812
     [Google Scholar]
  3. TangH. TangY. LiN. ShiQ. GuoJ. ShangE. DuanJ. Neuroprotective effects of scutellarin and scutellarein on repeatedly cerebral ischemia–reperfusion in rats.Pharmacol. Biochem. Behav.2014118515910.1016/j.pbb.2014.01.003 24423938
     [Google Scholar]
  4. DongZ.X. LiN.G. ZhangP.X. GuT. WuW.Y. ShiZ.H. An efficient chemical synthesis of scutellarein: An in vivo metabolite of scutellarin.Molecules201621326310.3390/molecules21030263 26927039
     [Google Scholar]
  5. AttimaradM. Mueen AhmedK.K. AldhubaibB.E. HarshaS. High-performance thin layer chromatography: A powerful analytical technique in pharmaceutical drug discovery.Pharm. Methods201122717510.4103/2229‑4708.84436 23781433
     [Google Scholar]
  6. YaoC. QiL. ZhongF. LiN. MaY. An integrated chemical characterization based on FT-NIR, GC–MS and LC-MS for the comparative metabolite profiling of wild and cultivated agarwood.J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.2022118812305610.1016/j.jchromb.2021.123056 34871920
     [Google Scholar]
  7. LiQ. YanX. ZhaoK. LiL. PengS. LuoX. WenY. YanZ. Fast inspection of saffron on the spot based on cloud-connected portable near-infrared technology.Guangpuxue Yu Guangpu Fenxi20204030293037
     [Google Scholar]
  8. ZhongF. ShenC. QiL. MaY. A multi-level strategy based on metabolic and molecular genetic approaches for the characterization of different coptis medicines using hplc-uv and rad-seq techniques.Molecules20182312309010.3390/molecules23123090 30486378
     [Google Scholar]
  9. QiL. ZhongF. LiuN. WangJ. NieK. TanY. MaY. XiaL. Characterization of the anti-AChE potential and alkaloids in Rhizoma Coptidis from different Coptis species combined with spectrum-effect relationship and molecular docking.Front. Plant Sci.202213102030910.3389/fpls.2022.1020309 36388527
     [Google Scholar]
  10. LiQ. QiL. ZhaoK. KeW. LiT. XiaL. Integrative quantitative and qualitative analysis for the quality evaluation and monitoring of Danshen medicines from different sources using HPLC-DAD and NIR combined with chemometrics.Front. Plant Sci.20221393285510.3389/fpls.2022.932855 36325569
     [Google Scholar]
  11. BholeR.P. ShindeS.S. ChitlangeS.S. WankhedeS.B. A high-performance thin layer chromatography (HPTLC) method for simultaneous determination of diphenhydramine hydrochloride and naproxen sodium in tablets. Anal. Chem. Insights, 201510ACI.S31506.10.4137/ACI.S3150626692760
     [Google Scholar]
  12. NeumannC. MargotP. New perspectives in the use of ink evidence in forensic science: Part I. Development of a quality assurance process for forensic ink analysis by HPTLC.Forensic Sci. Int.20091851-3293710.1016/j.forsciint.2008.11.016 19147310
     [Google Scholar]
  13. SyamasundarB. SuneethaA. Development and validation of HPTLC method for the estimation of almotriptan malate in tablet dosage form.Indian J. Pharm. Sci.201072562963210.4103/0250‑474X.78534 21694997
     [Google Scholar]
  14. KambojA. SalujaA.K. Development of validated HPTLC method for quantification of stigmasterol from leaf and stem of Bryophyllum pinnatum.Arab. J. Chem.201310.1016/j.arabjc.2013.10.006
     [Google Scholar]
  15. MathurR. Jayachandran NairC.V. AhamadS. KhanW. AnjumV. Development and validation of high-performance thin-layer chromatography method for simultaneous determination of polyphenolic compounds in medicinal plants.Pharmacognosy Res.201795Suppl. 16710.4103/pr.pr_122_16 29333046
     [Google Scholar]
  16. G, B.; Suripeddi, R.K. Development and validation of HPTLC method for identification and quantification of sterols from leaves of Erythroxylum monogynum Roxb. and in vitro evaluation of anti-oxidant and anti-glycation activities.S. Afr. J. Bot.2021137243410.1016/j.sajb.2020.10.005
     [Google Scholar]
  17. MajidA.M.S.A. HashimS. BehH.K. HamilM.S.R. IsmailZ. High-performance thin-layer chromatography method development, validation, and simultaneous quantification of four compounds identified in standardized extracts of Orthosiphon stamineus.Pharmacognosy Res.20168423824310.4103/0974‑8490.188872 27695261
     [Google Scholar]
  18. SreekanthN. AwenB. RaoC.B. HPTLC method development and validation of trandolapril in bulk and pharmaceutical dosage forms.J. Adv. Pharm. Technol. Res.20101217217910.4103/2231‑4040.72255 22247843
     [Google Scholar]
/content/journals/cpa/10.2174/0115734129312130240812070549
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Development and Validation of a High-performance Thin-layer Chromatography Method for Simultaneous Qualitative and Quantitative Analysis of Scutellarin and Scutellarein in S. barbata D. Don
Current Pharmaceutical Analysis 20, 597 (2024); https://doi.org/10.2174/0115734129312130240812070549
/content/journals/cpa/10.2174/0115734129312130240812070549
/content/journals/cpa/10.2174/0115734129312130240812070549
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  • Article Type:     Research Article
Keyword(s): HPTLC; method validation; quantitative analysis; S. barbata D. Don; scutellarein; scutellarin

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