Current Proteomics - Volume 19, Issue 3, 2022

Background: The exact mechanism of acute pancreatitis (AP), which is an inflammation of the pancreas, still remains unclear. Objective: In this study, we examined the protein phosphorylation changes during the early stage of AP in mice using proteomic analysis. Methods: AP model in mice was constructed using an intraperitoneal injection of cerulein. Blood samples and pancreas were collected at 1, 3, 6, 9h after the final inj Read More

Background: Amylase used in the market is mostly medium-temperature enzyme or high-temperature enzyme and has poor enzyme activity under low-temperature environment. Acid α-amylase can be used to develop digestion additives in the pharmaceutical and healthcare industries. The amino acid sequence and structural differences among α-amylases obtained from various organisms are high enough to confer interesti Read More

Background: Identification of a peripheral biological marker might aid in identifying patients at high risk of attempting suicide and might help in effective early intervention. Objective: In the present study, we extend the findings of our previous multidimensional proteomics study by examining the levels of plasma Apolipoprotein-AIV in patients diagnosed with major depression with and without suicidal ideation compared to age Read More

Background: H7N9 influenza virus poses a high risk to human beings, and proteomic evaluations of this virus may help better understand its pathogenic mechanisms in human systems. Objective: This study aimed at determining membrane proteins related to H7N9 infection. Methods: In this study, we infected primary human alveolar adenocarcinoma epithelial cells (A549) with H7N9 (including wild and mutant strains) an Read More

Background: Spiroplasma eriocheiris is a novel pathogen of freshwater crustaceans and is closely related to S. mirum. They have no cell wall and a helical morphology. They have the ability to infect mammals with an unclear mechanism. Objective: In this study, our aim was to investigate the profile of protein expression in 3T6 cells infected with S. eriocheiris. Methods: The proteome of 3T6 cells infected by S. eriocheiris was sy Read More

Objective: The tissue remodeling process and cellular migration relate to the activities of Matrix Metalloproteinases (MMPs). The aim of this study was to investigate the effects of a predicted motif from TIMPs on the MMP-2 and MMP-9 activities secreted from the differentiated macrophages. Materials and Methods: The monocytes were isolated from the healthy individuals by RosetteSep kit and were differentiated into macrop Read More

Aim: Proteomic profile analysis of pulmonary artery in a rat model under hypoxic pulmonary hypertension. Background: Hypoxic pulmonary hypertension (HPH) is a pathological condition exemplified by a constant rise in pulmonary artery pressure in high-altitudes. Objective: To investigated the proteome profile and response mechanisms of SD rats under hypoxia over a period of four-weeks. Methods: Proteomic Read More

Background: The absence of absolute clinical indicators and suitable biomarkers hinders the timely diagnosis of women at risk of preterm birth. It influences roughly 12% of births. At delivery and clinical presentation, preterm births are generally inspected based on the gestational period. Different disturbed pathways are associated with the signs of at-risk pregnancies. Objective: The main purpose of this study is to analyze Read More

Background: Preeclampsia (PE) is a common pregnancy-specific disease with potential adverse maternal and neonatal outcomes. Objective: We aimed to estimate proteomic profiles of serum-derived exosomes obtained from PE offspring with bioinformatics methods. Methods: Serum samples were collected from 12 h, 24 h, and 72 h newborns delivered by preeclamptic and normal pregnant women. Exosomes were ex Read More