Current Proteomics - Volume 11, Issue 4, 2014

Development of a transgenic animal model always is followed by extensive characterization steps. Here, we intended to test and optimize a fast screening method to get general findings about the differential protein composition in the heart of wild type (WT) and transgenic (TG) mice. Therefore, we developed a protocol for magnetic bead-based separation (MB) combined with Matrix-Assisted Laser Desorption/Ionizatio Read More

Proteins are the second largest portion of a cell after water, playing a wide variety of key roles in the cellular activities. Due to low-efficiency in determining protein functions, however, accurately and cost-effectively identifying functions of a protein is still one of major challenge in the post-genomics era. In an attempt to close this gap, we presented a novel neighborhood model to predict protein functions from protein-prote Read More

The water buffalo is a native species in Asia, and is considered to be a work animal. But because of an inherent defect, it has a low reproductive efficiency. This study presents research on the proteome of the water buffalo testis, a better understanding of which is very important for reproduction. 1-D SDS-PAGE and Reverse Phase Liquid chromatography mass spectrum (RPLC-MS/MS) were utilized, and 1383 proteins i Read More

The purpose of this research work was to investigate whether such a workflow including the phosphoproteins enriched by affinity purification, isolated and quantified by two-dimensional difference gel electrophoresis, the differential protein spots identified by mass spectrometry, was suitable to dissect the intact phosphoprotein profile that mediated cell signaling pathways. Endothelial cells with or without vascular end Read More

Lyme disease is caused by the spirochete Borrelia burgdorferi which enters into the skin by feeding ticks, mainly Ixodes species. Spread of the pathogen into the blood results in various symptoms. Because some individuals do not show any symptom following the infection, it is very difficult to diagnose the infection caused by this spirochete. When a proper treatment is not provided, this infection subsequently evolves to sev Read More

Under the environmental stress condition, protein folding or refolding has become an important issue for the survival of any microorganism. In majority of bacteria, such as Escherichia coli, temperature downshift leads to a transient arrest of cell growth, which results in inhibition of general protein synthesis. However, syntheses of a number of proteins, called cold-shock proteins are induced under these conditions. These pr Read More

Recent developments in bioinformatics have highlighted the importance of protein structure prediction for which information about structure classes forms the foundation and plays an important role in the prediction of protein folds and tertiary structure. The majority of previous researches have focused on only four protein classes in the Structure Classification of Proteins (SCOP) database. In this paper, we focus Read More