Current Proteomics - Volume 11, Issue 3, 2014

The NADPH-dependent reduction of glucose reaction that is catalyzed by Aldose Reductase (AR) follows a sequential ordered kinetic mechanism in which the co-factor NADPH binds to the enzyme prior to the aldehyde substrate. The kinetic/structural experiments have found a conformational change involving a hinge-like movement of a surface loop (residues 213-224) which is anticipated to take place upon the binding of the d Read More

Plant protein is seldom studied by researchers, particularly from the roots of Eurycoma longifolia. This is mainly due to the minute amount of plant protein and low sample recovery of protein extraction from the recalcitrant tissues. However, plant protein could be the bioactive constituent for numerous biological activities which have been proven scientifically and practicing traditionally. Therefore, four extraction methods rang Read More

Proteomics has advanced and identified various plasma proteins that may be implicated in lesions in brains from neurodegenerative individuals. Apolipoprotein E and amyloid beta are amongst the many candidate proteins identified in common neurodegenerative disorders with effects by diet and nutriproteomics on the interactions of apo E and amyloid beta. The purpose of this review paper is to specify the role of proteins an Read More

Tanshinone IIA is the major active ingredient of Salviae miltiorrhizae extract, and has been widely used in China for the treatment of cardiovascular and cerebrovascular diseases. We have identified proteins modulated by Tanshinone IIA during the formation of macrophage-derived foam cells to uncover its anti-atherosclerotic mechanism. Low density lipoprotein (LDL) isolated from hyperlipidemia patients, designated as HP-LDL. Read More

Epigenetic mechanisms orchestrate inheritable concerted networks essential for chromatin remodeling. Molecular interplays include post-translational modifications to histones, DNA methylation, activity of small non coding RNAs, govern activation and silencing of gene expression and define the molecular basis of pluripotency, reprogramming, early human development and differentiation. The implications of epigeneti Read More

The large collection of mutated genes in the cancer genome mapping onto a reduced set of signalling pathways highlights the paramount importance of signalling pathways in cancer therapeutics. Signalling proteins operate through a large and complex network associated with cell properties and ultimately reprogrammed in order to implement the known hallmark capabilities in cancer cells. Overall the underlying c Read More

Background: Stigmatic and stylar proteins play a pivotal role in plant reproduction during pollination and fertilization. Methods: Total proteins from kiwifruit (Actinidia. Arguta L.) stigmatic and stylar have been extracted in our present study at two time points, before pollination and 10 h after pollination. Further, proteins were characterized and identified using fluorescence differential in-gel electrophoresis (DIGE), matrix-assist Read More