
Full text loading...
We use cookies to track usage and preferences.I Understand
Hypothetical proteins (HPs) are those proteins whose functions are unknown; therefore, the present study was carried out to predict similarity-based functionality of HPs in selected bacteria Streptomyces coelicolor A3(2) and Neisseria meningitidis.
Annotation-based approaches using Pfam, orthology, String, Bi-directional Best Blast Hit, PSLpred, Subloc, Cello, homology modeling, and computational tools were used in evaluating the functionality of HPs.
Thirty-one domains in both bacterial species were retrieved based on the E-value score and compared with bacterial species already existing in databases. Statistical analysis was duly done to check which features performed well.
Out of 31 HPs found in Streptomyces coleicolor strain A3(2), 14 domains were found to be uncharacterized in their functionality, while 2 uncharacterized domains in the case of Neisseria meningitidis were assigned a function on similarity-based approaches. The annotation of HPs is a challenge in bacteria as these are based on the similarity of proteins in other species.
Article metrics loading...
Full text loading...
References
Data & Media loading...
Supplements