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Volume 21, Issue 3
  • ISSN: 1570-1646
  • E-ISSN: 1875-6247

Abstract

Background

Diabetic kidney disease (DKD) is a common microvascular complication of diabetic mellitus (DM). At present, the early diagnosis of DKD mainly depends on microalbuminuria, which is prone to be affected by confounding factors such as urinary tract infections.

Methods

To identify the more stable early diagnosis markers, the whole proteome in the circulating exosomes from controls, DM patients, and DKD patients was quantified by label-free proteomics analysis and then validated with parallel reaction monitoring.

Results

Three hundred ninety-one quantitative proteins were detected, and the expression trends of 7 proteins in the validation phase were consistent with that in the discovery phase. The expression level assessment results revealed that the expression of EFEMP1 and ApoA4 in the DKD group was significantly higher than those in DM and controls. Correlation analysis showed that EFEMP1 and APOA4 were positively correlated with urinary microalbumin and urinary albumin creatinine ratio and had excellent diagnostic values for distinguishing DKD from DM and controls.

Conclusion

ApoA4 and EFEMP1 could serve as the early diagnosis markers of DKD. These findings provide a possibility for the development of a clinical diagnostic index that can efficiently distinguish DKD from DM in the near future.

© 2024 Bentham Science Publishers
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2024-09-01
2025-06-23

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/content/journals/cp/10.2174/0115701646309538240805093732
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Circulating Exosomes Studied by Label-free Proteomics Analysis Reveal Transition Signatures from Diabetes Mellitus to Diabetic Kidney Disease
Curr. Proteomics 21, 187 (2024); https://doi.org/10.2174/0115701646309538240805093732
/content/journals/cp/10.2174/0115701646309538240805093732
/content/journals/cp/10.2174/0115701646309538240805093732
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  • Article Type:     Research Article
Keyword(s): diabetic mellitus; differentially expressed proteins; exosome; label-free proteomics analysis; parallel reaction monitoring; Type 2 diabetic kidney disease

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