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Volume 20, Issue 1
  • ISSN: 1573-4080
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Abstract

Enzymatic browning is a negative change that affects fruits and vegetables. This makes them unfit for consumption or reduces their shelf life and quality. This reaction alters the appearance, texture, fragrance, taste, and nutritional value of the food. In order for these products to be widely accepted by consumers, it is necessary to use natural compounds with anti-browning properties.

Objective

The main objective of this study was to select effective extracts for the anti-browning (anti-polyphenol oxidase and anti-peroxidase), and antioxidant activities of by-products of L., Ghars variety, from Algeria.

Methods

Hydro-methanolic extracts from the pedicel, perianth, and leaves of date palm were examined to evaluate the phenol and total flavonoid contents. Using spectrophotometric techniques, the antioxidant activities were assessed using 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH●), 2, 2’-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) radical (ABTS●+) and Ferric Reducing Antioxidant Power (FRAP) assay, examined the enzyme inhibitory activity against polyphenol oxidase and peroxidase of L. extract.

Results

The range of total phenolic and flavonoid contents was 12.29 to 48.98 mg gallic acid equivalent/g dry matter and 2.83 to 15.07 mg rutin equivalent/g dry matter, respectively. The pedicel extracts showed significant antioxidant activity in the DPPH and FRAP tests (IC = 0.0057 ± 0.0010 mg/ml and FEAC = 1.1961 ± 0.0647) compared to other extracts. However, in the ABTS assay, the leaf extract exhibited an interesting potency (IC = 0.0020 ± 0.0001mg/ml). The study on the anti-browning activity of date palm by-product extracts showed that it inhibited the activity of peroxidase enzyme from date palm fruit.

Conclusion

This work is the first time the potential of an extract from date palm by-products to enzymatically reduce the browning of date palms is presented. According to the results obtained, the different organs studied from the Ghars date palm, are a powerful natural antioxidant and may include natural compounds that retard browning by enzymes.

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2024-03-01
2024-11-16

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References

  1. Ahmad Mohd ZainM.R. Abdul KariZ. DawoodM.A.O. Bioactivity and pharmacological potential of date palm (phoenix dactylifera l.) against pandemic COVID-19: A comprehensive review.Appl. Biochem. Biotechnol.2022194104587462410.1007/s12010‑022‑03952‑2 35579740
     [Google Scholar]
  2. Al-AlawiR.A. Al-MashiqriJ.H. Al-NadabiJ.S.M. Al-ShihiB.I. BaqiY. Date palm tree (Phoenix dactylifera L.): natural products and thera-peutic options.Front Plant Sci2017884510.3389/fpls.2017.00845 28588600
     [Google Scholar]
  3. RekisA. LaiadiZ. MehenniM. Morphological characteristics denomination of date palm studied cultivars.AJAE2020141131140
     [Google Scholar]
  4. Ali Al-ShuraymL. The impact of the onion-garlic extracts to control date palm aphids in Saudi Arabia.J. Saudi Soc. Agric. Sci.202221854655110.1016/j.jssas.2022.03.004
     [Google Scholar]
  5. BenouamaneO. Vergara-BarberánM. BenazizaA. Characterization of different cultivars of Algerian date palm (Phoenix dactylifera L.) leaves and pollen by comprehensive two-dimensional liquid chromatography of phenolic compounds extracted with different sol-vents.Microchem. J.202218210787410.1016/j.microc.2022.107874
     [Google Scholar]
  6. BakouriZ.E. MezianiR. MazriM.A. ChittM.A. BouamriR. JaitiF. Estimation of the production cost of date fruits of cultivar majhoul (Phoenix dactylifera L.) and evaluation of the Moroccan competitiveness towards the major exporting regions in the world.Agric. Sci.202112111342135110.4236/as.2021.1211086
     [Google Scholar]
  7. ZihadS.M.N.K. UddinS.J. SifatN. Antioxidant properties and phenolic profiling by UPLC-QTOF-MS of Ajwah, Safawy and Sukkari cultivars of date palm.Biochem. Biophys. Rep.20212510090910.1016/j.bbrep.2021.100909 33521336
     [Google Scholar]
  8. SarrafM. JemniM. KahramanoğluI. Commercial techniques for preserving date palm (Phoenix dactylifera) fruit quality and safety: A review.Saudi J. Biol. Sci.20212884408442010.1016/j.sjbs.2021.04.035 34354425
     [Google Scholar]
  9. Al-AmraniM. Al-AlawiA. Al-MarhobiI. Assessment of enzymatic browning and evaluation of antibrowning methods on dates.Int. J. Food Sci.202020201910.1155/2020/8380461 32190643
     [Google Scholar]
  10. HamdanN. LeeC.H. WongS.L. FauziC.E.N.C.A. ZamriN.M.A. LeeT.H. Prevention of enzymatic browning by natural extracts and genome-editing: A review on recent progress.Molecules2022273110110.3390/molecules27031101 35164369
     [Google Scholar]
  11. MoonK.M. KwonE.B. LeeB. KimC.Y. Recent trends in controlling the enzymatic browning of fruit and vegetable products.Molecules20202512275410.3390/molecules25122754 32549214
     [Google Scholar]
  12. ChandrasekharS. Studying the rate of polyphenol oxidase activity in apples using a colorimeter.Open Sci202061
     [Google Scholar]
  13. QiaoL. WangH. ShaoJ. LuL. TianJ. LiuX. A novel mitigator of enzymatic browning—hawthorn leaf extract and its application in the preservation of fresh-cut potatoes.Food Qual Saf.20215fyab015
     [Google Scholar]
  14. KaewjumpolG. SrisamleeS. BecklesD.M. LuengwilaiK. Enzymatic browning in banana blossoms and techniques for its reduction.Horticulturae202171037310.3390/horticulturae7100373
     [Google Scholar]
  15. DiasC. FonsecaA.M.A. AmaroA.L. Natural-based antioxidant extracts as potential mitigators of fruit browning.Antioxidants20209871510.3390/antiox9080715 32784698
     [Google Scholar]
  16. IoannouI. Prevention of enzymatic browning in fruit and vegetables.Eur. Sci. J.2013930
     [Google Scholar]
  17. DouY. ChangC. WangJ. Hydrogen sulfide inhibits enzymatic browning of fresh-cut Chinese water chestnuts.Front. Nutr.2021865298410.3389/fnut.2021.652984 34150826
     [Google Scholar]
  18. VenturiF. BartoliniS. SanmartinC. Potato peels as a source of novel green extracts suitable as antioxidant additives for fresh-cut fruits.Appl. Sci.2019912243110.3390/app9122431
     [Google Scholar]
  19. DjeridaneA. YousfiM. NadjemiB. MaamriS. DjirebF. StockerP. Phenolic extracts from various Algerian plants as strong inhibitors of porcine liver carboxylesterase.J. Enzyme Inhib. Med. Chem.200621671972610.1080/14756360600810399 17252945
     [Google Scholar]
  20. SingletonV.L. RossiJ.A.Jr Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents.Am. J. Enol. Vitic.196516314415810.5344/ajev.1965.16.3.144
     [Google Scholar]
  21. LamaisonJ.L. CarnatA. Teneur en principaux flavonoïdes des fleurs et des feuilles de Crataegus monogyna Jacq. et de Crataegus laevigita (Poiret) DC.(Rosaceae).Pharm. Acta Helv.199165315320
     [Google Scholar]
  22. LeeS.K. MbwamboZ.H. ChungH. Evaluation of the antioxidant potential of natural products.Comb. Chem. High Throughput Screen.199811354610.2174/138620730101220118151526 10499128
     [Google Scholar]
  23. CanoA. Hernández‐RuízJ. García‐CánovasF. AcostaM. ArnaoM.B. An end‐point method for estimation of the total antioxidant activity in plant material. Phytochemical Analysis.International Journal of Plant Chemical and Biochemical Techniques199894196202
     [Google Scholar]
  24. BenzieI.F.F. StrainJ.J. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay.Anal. Biochem.19962391707610.1006/abio.1996.0292 8660627
     [Google Scholar]
  25. Al-JassabiS. SaadA. SatyakeerthyT.R. AbdullahM.S. Characterization of polyphenol oxidase from Zyzyphus spina-christi from Iraq.Middle East J. Sci. Res.2013142155160
     [Google Scholar]
  26. LeeM. LeeM. ParkI. Inhibitory effect of onion extract on polyphenol oxidase and enzymatic browning of taro (Colocasia antiquorum var. esculenta).Food Chem.2007105252853210.1016/j.foodchem.2007.04.010
     [Google Scholar]
  27. PonceA.G. del ValleC.E. RouraS.I. Natural essential oils as reducing agents of peroxidase activity in leafy vegetables.Lebensm. Wiss. Technol.200437219920410.1016/j.lwt.2003.07.005
     [Google Scholar]
  28. MessaoudiA. DekmoucheM. RahmaniZ. BensaciC. Phenolic profile, Antioxidant potential of date (Phoenix dactylifera Var. Degla Baidha and Deglet-Nour) seeds from Debila region (Oued Souf, Algeria).Asian J. Res. Chem20211411510.5958/0974‑4150.2021.00006.7
     [Google Scholar]
  29. LaouiniS.E. Phytochemical study and biological activity of extract of leaves of Phoenix dactylifera L. in the southern region of Algeria (the region of Oued Souf.In: PhD Thesis, Mohamed Khider Biskra University. 2014
     [Google Scholar]
  30. DibactoR.E.K. TchuenteB.R.T. NguedjoM.W. Total polyphenol and flavonoid content and antioxidant capacity of some varieties of Persea americana peels consumed in Cameroon.Scientific World Journal2021202111110.1155/2021/8882594 33976588
     [Google Scholar]
  31. MessaoudiR. AbbeddouS. MansouriA. CalokerinosA.C. KefalasP. Phenolic profile and antioxidant activity of date-pits of seven Algeri-an date palm fruit varieties.Int. J. Food Prop.20131651037104710.1080/10942912.2011.576355
     [Google Scholar]
  32. ZinebG. BoukouadaM. DjeridaneA. SaidiM. YousfiM. Screening of antioxidant activity and phenolic compounds of various date palm (Phoenix dactylifera) fruits from Algeria.Med. J. Nutrition Metab.20125211912610.1007/s12349‑011‑0082‑7
     [Google Scholar]
  33. ManssouriM. ZniniM. MajidiL. Studies on the antioxidant activity of essential oil and various extracts of Ammodaucus leucotrichus Coss. & Dur. Fruits from Morocco.J. Taibah Univ. Sci.202014112413010.1080/16583655.2019.1710394
     [Google Scholar]
  34. KedareS.B. SinghR.P. Genesis and development of DPPH method of antioxidant assay.J. Food Sci. Technol.201148441242210.1007/s13197‑011‑0251‑1 23572765
     [Google Scholar]
  35. LoganayakiN. SiddhurajuP. ManianS. Antioxidant activity and free radical scavenging capacity of phenolic extracts from Helicteres isora L. and Ceiba pentandra L.J. Food Sci. Technol.201350468769510.1007/s13197‑011‑0389‑x 24425970
     [Google Scholar]
  36. RaghavendraH.L. PrashithK.T.R. AkarshS.M. AshwiniH.S. Phytochemical analysis, antifungal and antioxidant activity of leaf and fruit of Zizyphus xylopyrus (Retz.) Willd.(Rhamnaceae).Sci. Technol. Arts Res. J.201644838810.4314/star.v4i4.12
     [Google Scholar]
  37. KekudaTRP RaghavendraHL SolomonT DuressaD Antifungal and antiradical potential of Moringa stenopetala (Baker f.) Cufod (Moringaceae).J biosci agric res2016111923910.18801/jbar.110116.112
     [Google Scholar]
  38. ChristodoulouM.C. Orellana PalaciosJ.C. HesamiG. Spectrophotometric methods for measurement of antioxidant activity in food and pharmaceuticals.Antioxidants20221111221310.3390/antiox11112213 36358583
     [Google Scholar]
  39. SudanR. BhagatM. GuptaS. SinghJ. KoulA. Iron (FeII) chelation, ferric reducing antioxidant power, and immune modulating potential of Arisaema jacquemontii (Himalayan Cobra Lily).BioMed Res. Int.201420141710.1155/2014/179865 24895548
     [Google Scholar]
  40. TaşkinD. Geçi̇mM. DoğanA. BecerenA. Polyphenolic composition and antioxidant effect of aerial parts and roots extracts from Scorzon-era veratrifolia.Int J Second Metab20218328429910.21448/ijsm.943707
     [Google Scholar]
  41. IslamM.Z. HossainM.T. HossenF. MukharjeeS.K. SultanaN. PaulS.C. Evaluation of antioxidant and antibacterial activities of Crotalaria pallida stem extract.Clin Phytoscience201841810.1186/s40816‑018‑0066‑y
     [Google Scholar]
  42. FaroukB. ArefN. RachidC. Characterization of three polyphenol oxidase isoforms in royal dates and inhibition of its enzymatic browning reaction by indole-3-acetic acid.Int. J. Biol. Macromol.202014589490310.1016/j.ijbiomac.2019.09.140 31770554
     [Google Scholar]
  43. BenaceurF. GouziH. MeddahB. NeifarA. GuergouriA. Purification and characterization of catechol oxidase from tadela (Phoenix dac-tylifera L.) date fruit.Int. J. Biol. Macromol.20191251248125610.1016/j.ijbiomac.2018.09.101 30236755
     [Google Scholar]
  44. McDonaldA.G. TiptonK.F. Parameter reliability and understanding enzyme function.Molecules202227126310.3390/molecules27010263 35011495
     [Google Scholar]
  45. GouziH. BenmansourA. Partial purification and characterization of polyphenol oxidase extracted from Agaricus bisporus (JE Lange) Imbach.Int. J. Chem. React. Eng.20075110.2202/1542‑6580.1445
     [Google Scholar]
  46. RamsayR. TiptonK. Assessment of enzyme inhibition: A review with examples from the development of monoamine oxidase and cho-linesterase inhibitory drugs.Molecules2017227119210.3390/molecules22071192 28714881
     [Google Scholar]
  47. BukerS M Boriack-SjodinP A CopelandR A Enzyme–inhibitor interactions and a simple, rapid method for determining inhibition mo-dality.SLAS DISCOVERY: Advancing Life Sciences R&D,20192455152210.1177/2472555219829898
     [Google Scholar]
  48. YadavG.D. MagadumD.B. Kinetic modelling of enzyme catalyzed biotransformation involving activations and inhibitions, Croatia: In Tech.201773124
     [Google Scholar]
  49. JohnJ.A. ShahidiF. Phenolic content, antioxidant and anti-inflammatory activities of seeds and leaves of date palm (Phoenix dactylifera L.).J. Food Bioact.2019512013010.31665/JFB.2019.5179
     [Google Scholar]
  50. Abu-ReidahI.M. Gil-IzquierdoÁ. MedinaS. FerreresF. Phenolic composition profiling of different edible parts and by-products of date palm (Phoenix dactylifera L.) by using HPLC-DAD-ESI/MSn.Food Res. Int.2017100Pt 349450010.1016/j.foodres.2016.10.018 28964373
     [Google Scholar]
  51. LimW.Y. WongC.W. Inhibitory effect of chemical and natural anti-browning agents on polyphenol oxidase from ginger (Zingiber offici-nale Roscoe).J. Food Sci. Technol.20185583001300710.1007/s13197‑018‑3218‑7 30065409
     [Google Scholar]
  52. LiuX. ChenT. WangQ. LiuJ. LuY. ShiY. Structure analysis and study of biological activities of condensed tannins from Bruguiera gymnorhiza (L.) Lam and their effect on fresh-cut lotus roots.Molecules2021265136910.3390/molecules26051369 33806398
     [Google Scholar]
  53. BaltasN. PakyildizS. CanZ. DincerB. KolayliS. Biochemical properties of partially purified polyphenol oxidase and phenolic com-pounds of Prunus spinosa L. subsp. dasyphylla as measured by HPLC-UV.Int. J. Food Prop.201720S1115
     [Google Scholar]
/content/journals/cei/10.2174/1573408019666230807161244
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Natural Antioxidant Extracts as Anti-browning Agents from Algerian Date Palm Fruit
Current Enzyme Inhibition 20, 20 (2024); https://doi.org/10.2174/1573408019666230807161244
/content/journals/cei/10.2174/1573408019666230807161244
/content/journals/cei/10.2174/1573408019666230807161244
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  • Article Type:     Research Article
Keyword(s): enzymatic browning; antioxidant activity; by-products; peroxidase; Phoenix dactylifera L.; polyphenol oxidase

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